Elevated concentration and biologic activity of monocyte chemotactic protein-1 levels in the peritoneal fluid of patients with endometriosis

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dc.contributor.authorAkoum, A.
dc.contributor.authorLemay, A.
dc.contributor.authorMcColl, S.
dc.contributor.authorTurcot-Tremblay, L.
dc.contributor.authorMaheux, R.
dc.date.issued1996
dc.description.abstract<h4>Objective</h4>To estimate the concentration and the biologic activity of monocyte chemotactic protein-1 (MCP-1) in the peritoneal fluid (PF) of women with and without endometriosis.<h4>Design</h4>A case control study was conducted.<h4>Setting</h4>Gynecology clinic and Laboratories of endocrinology of reproduction and immunology.<h4>Patients</h4>Women presenting for infertility, pelvic pain, or tubal ligation in which endometriosis was diagnosed at laparoscopy (n = 36) and normal fertile controls presenting for tubal ligation (n = 21).<h4>Interventions</h4>Collection of PF via laparoscopy.<h4>Main outcome measures</h4>Determination of PF concentrations of MCP-1 by an ELISA and evaluation of its monocyte chemotactic activity using a human hystiocytic cell line (U937). RESULTS. The concentration of MCP-1 (median, range of values) was increased in the PF of endometriosis patients (283, 0 to 1,930 pg/mL; conversion factor to SI unit, 0.155) compared with the control group (140, 0 to 435 pg/mL). The most significant elevation of MCP-1 levels was found in the stage II of the disease (371, 200 to 1,930 pg/mL). An increased chemotactic activity for monocytes (mean number of migrating cells/mm2 +/- SD) also was found in stages I (1,460 +/- 312) and II (1,541 +/- 336) of the disease when compared with fertile controls (393 +/- 56). Forty percent to 53% of this activity was inhibited in the presence of an antibody specific to MCP-1.<h4>Conclusions</h4>These observations are consistent with previous data indicating increased leukocyte chemotaxis in the PF of patients with endometriosis and suggest that MCP-1 may play a relevant role in the peritoneal inflammatory reaction associated with the disease.
dc.identifier.citationFertility and Sterility, 1996; 66(1):17-23
dc.identifier.doi10.1016/s0015-0282(16)58381-2
dc.identifier.issn0015-0282
dc.identifier.issn1556-5653
dc.identifier.orcidMcColl, S. [0000-0003-0949-4660]
dc.identifier.urihttp://hdl.handle.net/2440/11561
dc.language.isoen
dc.publisherElsevier BV
dc.source.urihttps://doi.org/10.1016/s0015-0282(16)58381-2
dc.subjectMonocytes
dc.subjectAscitic Fluid
dc.subjectHumans
dc.subjectEndometriosis
dc.subjectCase-Control Studies
dc.subjectChemotaxis, Leukocyte
dc.subjectOsmolar Concentration
dc.subjectAdult
dc.subjectFemale
dc.subjectChemokine CCL2
dc.titleElevated concentration and biologic activity of monocyte chemotactic protein-1 levels in the peritoneal fluid of patients with endometriosis
dc.typeJournal article
pubs.publication-statusPublished

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