An optical fiber based immunosensor for localized detection of IL-1β in rat spinal cord

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dc.contributor.authorZhang, K.
dc.contributor.authorArman, A.
dc.contributor.authorAnwer, A.
dc.contributor.authorHutchinson, M.
dc.contributor.authorGoldys, E.
dc.date.issued2019
dc.description.abstractSensitive and localized measurements of cytokines is important in biomedicine as cytokines are produced locally where needed to induce an immune reaction. Here, we present a novel immunosensor deposited on the optical fiber surface. The sensor is capable of localized detection of interleukin-1beta (IL-1β) in the rat spinal cord. In this immunosensor, a stable immunocapture surface was formed via a biotin-streptavidin coupling strategy and fluorescent carboxylated supermagnetic iron oxide (SPIO)-IL-1β detection antibody conjugates were used for signal amplification. Under the optimal condition, the proposed immunosensor can be used for the estimation of IL-1β in vitro in the range from 3.13 pg.mL⁻¹ to 400 pg.mL⁻¹ with a detection limit of 1.12 pg.mL⁻¹. Furthermore, the performance of the fiber sensor was firstly assessed by ex-vivo monitoring the secretions of the rat macrophages stimulated by lipopolysaccharide (LPS), and the results demonstrated significant correlations with a commercial ELISA kit. Furthermore, the fiber sensor was successfully utilized to carry out a localized measurement of IL-1β in a spinal cord of an anesthetized rat. The result indicates that such fiber sensors can be used as an effective and sensitive tool for localized detection of IL-1β in vivo, in a range of research and clinical applications.
dc.description.statementofresponsibilityKaixin Zhang, Azim Arman, Ayad G. Anwer, Mark R. Hutchinson, Ewa M. Goldys
dc.identifier.citationSensors and Actuators B: Chemical: international journal devoted to research and development of physical and chemical transducers, 2019; 282:122-129
dc.identifier.doi10.1016/j.snb.2018.11.054
dc.identifier.issn0925-4005
dc.identifier.orcidHutchinson, M. [0000-0003-2154-5950]
dc.identifier.urihttp://hdl.handle.net/2440/118324
dc.language.isoen
dc.publisherElsevier
dc.relation.granthttp://purl.org/au-research/grants/arc/CE140100003
dc.rights© 2018 Published by Elsevier B.V.
dc.source.urihttps://doi.org/10.1016/j.snb.2018.11.054
dc.subjectOptical fiber; interleukin-1beta; in vivo; sensor; immunosensor
dc.titleAn optical fiber based immunosensor for localized detection of IL-1β in rat spinal cord
dc.title.alternativeAn optical fiber based immunosensor for localized detection of IL-1beta in rat spinal cord
dc.typeJournal article
pubs.publication-statusPublished

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