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An improved culture method for selective isolation of Campylobacter jejuni from wastewater

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dc.contributor.authorKim, J.
dc.contributor.authorOh, E.
dc.contributor.authorBanting, G.S.
dc.contributor.authorBraithwaite, S.
dc.contributor.authorChui, L.
dc.contributor.authorAshbolt, N.J.
dc.contributor.authorNeumann, N.F.
dc.contributor.authorJeon, B.
dc.date.issued2016
dc.description.abstractCampylobacter jejuni is one of the leading foodborne pathogens worldwide. C. jejuni is isolated from a wide range of foods, domestic animals, wildlife, and environmental sources. The currently available culture-based isolation methods are not highly effective for wastewater samples due to the low number of C. jejuni in the midst of competing bacteria. To detect and isolate C. jejuni from wastewater samples, in this study, we evaluated a few different enrichment conditions using five different antibiotics (i.e., cefoperazone, vancomycin, trimethoprim, polymyxin B, and rifampicin), to which C. jejuni is intrinsically resistant. The selectivity of each enrichment condition was measured with Ct value using quantitative real-time PCR, and multiplex PCR to determine Campylobacter species. In addition, the efficacy of Campylobacter isolation on different culture media after selective enrichment was examined by growing on Bolton and Preston agar plates. The addition of polymyxin B, rifampicin, or both to the Bolton selective supplements enhanced the selective isolation of C. jejuni. The results of 16S rDNA sequencing also revealed that Enterococcus spp. and Pseudomonas aeruginosa are major competing bacteria in the enrichment conditions. Although it is known to be difficult to isolate Campylobacter from samples with heavy contamination, this study well exhibited that the manipulation of antibiotic selective pressure improves the isolation efficiency of fastidious Campylobacter from wastewater.
dc.identifier.citationFrontiers in Microbiology, 2016; 7(1345):1-8
dc.identifier.doi10.3389/fmicb.2016.01345
dc.identifier.issn1664-302X
dc.identifier.issn1664-302X
dc.identifier.urihttps://hdl.handle.net/11541.2/36639
dc.language.isoen
dc.publisherFrontiers Research Foundation
dc.rightsCopyright 2016 The author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/)
dc.source.urihttps://doi.org/10.3389/fmicb.2016.01345
dc.subjectantibiotics
dc.subjectcampylobacter jejuni
dc.subjectisolation
dc.titleAn improved culture method for selective isolation of Campylobacter jejuni from wastewater
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9916805629901831

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