Regulation of transcripts encoding ADAMTS-1 (a disintegrin and metalloproteinase with thrombospondin-like motifs-1) and progesterone receptor by human chorionic gonadotropin in equine preovulatory follicles

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dc.contributor.authorBoerboom, D.
dc.contributor.authorRussell, D.
dc.contributor.authorRichards, J.
dc.contributor.authorSirois, J.
dc.date.issued2003
dc.description.abstractOne member of a new family of metalloproteinases, a disintegrin and metalloproteinase with thrombospondin-like motifs-1 (ADAMTS-1), has been found to be expressed and hormonally induced in granulosa cells of ovulating rodent follicles. Furthermore, the targeted disruption of the ADAMTS-1 gene resulted in ovarian defects associated with severely impaired fertility. While these data demonstrate the importance of ADAMTS-1 in rodent ovarian physiology, the potential role of ADAMTS-1 in the ovulatory process of monoovulatory species remains unknown. The objectives of this study were to clone the equine ADAMTS-1 primary transcript and to study its regulation during human chorionic gonadotropin (hCG)-induced ovulation. A 3573 bp follicular cDNA library clone was isolated and found to encode a nearly complete, highly conserved ADAMTS-1 homologue. Real-time RT-PCR analysis detected this transcript in diverse tIssues, including previously unreported sites of ADAMTS-1 expression such as the male reproductive tract, the follicular theca interna and the mature corpus luteum. The tIssue distribution of the progesterone receptor (PR), a known regulator of ADAMTS-1 expression in rodent preovulatory follicles, was found to overlap that of ADAMTS-1 in some tIssues. A study of the regulation of follicular ADAMTS-1 and PR mRNAs during the hCG-induced ovulatory process revealed distinct patterns of regulation in granulosa cells and in theca interna. In granulosa cells, ADAMTS-1 mRNA was found to be induced at 12 h post-hCG (P<0.05), followed by a return to basal levels by 30 h and a re-increase at 33-39 h (P<0.05). A concomitant increase in PR mRNA (P<0.05) was observed at 12 h post-hCG. In theca interna, abundant ADAMTS-1 mRNA was detected at all timepoints, and levels increased transiently at 33 h post-hCG (P<0.05), whereas no significant change was observed in PR mRNA. Together, these data demonstrate for the first time the hormonally regulated ovarian expression of ADAMTS-1 in a monoovulatory species, and identify a novel biphasic regulation of ADAMTS-1 in granulosa cells and a regulated expression in theca interna that were not previously observed in rodents.
dc.description.statementofresponsibilityD Boerboom, DL Russell, JS Richards, and J Sirois
dc.identifier.citationJournal of Molecular Endocrinology, 2003; 31(3):473-485
dc.identifier.doi10.1677/jme.0.0310473
dc.identifier.issn0952-5041
dc.identifier.issn1479-6813
dc.identifier.orcidRussell, D. [0000-0002-4930-7658]
dc.identifier.urihttp://hdl.handle.net/2440/37472
dc.language.isoen
dc.publisherSoc Endocrinology
dc.rights© 2003 by Society for Endocrinology
dc.source.urihttps://doi.org/10.1677/jme.0.0310473
dc.subjectCorpus Luteum
dc.subjectGranulosa Cells
dc.subjectTheca Cells
dc.subjectAnimals
dc.subjectHorses
dc.subjectHumans
dc.subjectRats
dc.subjectChorionic Gonadotropin
dc.subjectMetalloendopeptidases
dc.subjectDisintegrins
dc.subjectReceptors, Progesterone
dc.subjectGene Expression Regulation
dc.subjectAmino Acid Sequence
dc.subjectBase Sequence
dc.subjectOvulation
dc.subjectGene Library
dc.subjectMolecular Sequence Data
dc.subjectFemale
dc.subjectMale
dc.subjectADAM Proteins
dc.subjectADAMTS1 Protein
dc.titleRegulation of transcripts encoding ADAMTS-1 (a disintegrin and metalloproteinase with thrombospondin-like motifs-1) and progesterone receptor by human chorionic gonadotropin in equine preovulatory follicles
dc.typeJournal article
pubs.publication-statusPublished

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