Production of fertile regenerants from protoplasts of Triticum tauschii (Coss.) Schmal.
| dc.contributor.author | Afshar-Sterle, S. | |
| dc.contributor.author | Kollmorgen, J. | |
| dc.contributor.author | Fincher, G. | |
| dc.date.issued | 2000 | |
| dc.description.abstract | <jats:p> Four suspension cell lines generated from two accessions of Triticum tauschii (Coss.) Schmal. (Aegilops squarrosa, 2n = 2x = 14, DD genome) were used to develop an efficient protocol for producing fertile regenerants from protoplasts. Protoplasts were isolated from each cell line by incubating fine cell aggregates (<500 µm in diameter) in a solution containing 3% Cellulase ‘Onozuka’ RS, 0.5% Macerozyme R10 and 0.2% Pectolyase Y23. Cell division occurred when the protoplasts were cultured at a density of 1.0–1.5 x 106 protoplasts mL–1 in half-strength MS medium supplemented with 1.1 mg L–1 2,4-dichlorophenoxyacetic acid (2,4-D), 0.6 M glucose and 1.2% agarose. The first cell divisions were observed after 5–7 days. Cell colonies were observed after 14 days and these grew quickly into large clumps when transferred to half-strength MS medium supplemented with 2.2 mg L-1 2,4-D, 30 g L–1 sucrose and solidified with 0.25% Phytagel. The colonies produced somatic embryos within 21–28 days of transfer to this medium. Somatic embryos were transferred to hormone-free MS medium for regeneration into plantlets. Although many regenerants produced shrivelled seeds, 9 of 16 were fertile and produced normal seeds.</jats:p> | |
| dc.identifier.citation | Australian Journal of Botany, 2000; 48(4):501-506 | |
| dc.identifier.doi | 10.1071/BT99014 | |
| dc.identifier.issn | 0067-1924 | |
| dc.identifier.issn | 1444-9862 | |
| dc.identifier.uri | http://hdl.handle.net/2440/13346 | |
| dc.language.iso | en | |
| dc.publisher | C S I R O Publishing | |
| dc.source.uri | https://doi.org/10.1071/bt99014 | |
| dc.title | Production of fertile regenerants from protoplasts of Triticum tauschii (Coss.) Schmal. | |
| dc.type | Journal article | |
| pubs.publication-status | Published |