The apical caspase dronc governs programmed and unprogrammed cell death in Drosophila

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dc.contributor.authorChew, S.
dc.contributor.authorAkdemir, F.
dc.contributor.authorChen, P.
dc.contributor.authorLu, W.
dc.contributor.authorMills, K.
dc.contributor.authorDaish, T.
dc.contributor.authorKumar, S.
dc.contributor.authorRodriguez, A.
dc.contributor.authorAbrams, J.
dc.date.issued2004
dc.description.abstractAmong the seven caspases encoded in the fly genome, only dronc contains a caspase recruitment domain. To assess the function of this gene in development, we produced a null mutation in dronc. Animals lacking zygotic dronc are defective for programmed cell death (PCD) and arrest as early pupae. These mutants present a range of defects, including extensive hyperplasia of hematopoietic tissues, supernumerary neuronal cells, and head involution failure. dronc genetically interacts with the Ced4/Apaf1 counterpart, Dark, and adult structures lacking dronc are disrupted for fine patterning. Furthermore, in diverse models of metabolic injury, dronc− cells are completely insensitive to induction of cell killing. These findings establish dronc as an essential regulator of cell number in development and illustrate broad requirements for this apical caspase in adaptive responses during stress-induced apoptosis.
dc.description.statementofresponsibilitySu Kit Chew, Fatih Akdemir, Po Chen, Wan-Jin Lu, Kathryn Mills, Tasman Daish, Sharad Kumar, Antony Rodriguez and John M. Abrams
dc.identifier.citationDevelopmental Cell, 2004; 7(6):897-907
dc.identifier.doi10.1016/j.devcel.2004.09.016
dc.identifier.issn1534-5807
dc.identifier.issn1878-1551
dc.identifier.orcidKumar, S. [0000-0001-7126-9814]
dc.identifier.urihttp://hdl.handle.net/2440/9400
dc.language.isoen
dc.publisherCell Press
dc.source.urihttp://www.developmentalcell.com/
dc.subjectEye
dc.subjectHemocytes
dc.subjectAnimals
dc.subjectDrosophila melanogaster
dc.subjectCaspases
dc.subjectDrosophila Proteins
dc.subjectGreen Fluorescent Proteins
dc.subjectRNA, Messenger
dc.subjectImmunohistochemistry
dc.subjectGenetic Complementation Test
dc.subjectIn Situ Nick-End Labeling
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectCell Death
dc.subjectApoptosis
dc.subjectGene Expression Regulation, Developmental
dc.subjectMutagenesis
dc.subjectBody Patterning
dc.subjectGenotype
dc.subjectHomozygote
dc.subjectMutation
dc.subjectAlleles
dc.subjectGenome
dc.subjectModels, Genetic
dc.subjectTime Factors
dc.subjectImage Processing, Computer-Assisted
dc.titleThe apical caspase dronc governs programmed and unprogrammed cell death in Drosophila
dc.typeJournal article
pubs.publication-statusPublished

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