Lanthanide-containing polymer microspheres by multiple-stage dispersion polymerization for highly multiplexed bioassays

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dc.contributor.authorAbdelrahman, A.
dc.contributor.authorDai, S.
dc.contributor.authorThickett, S.
dc.contributor.authorOrnatsky, O.
dc.contributor.authorBandura, D.
dc.contributor.authorBaranov, V.
dc.contributor.authorWinnik, M.
dc.date.issued2009
dc.description.abstractWe describe the synthesis and characterization of metal-encoded polystyrene microspheres by multiple-stage dispersion polymerization with diameters on the order of 2 mum and a very narrow size distribution. Different lanthanides were loaded into these microspheres through the addition of a mixture of lanthanide salts (LnCl(3)) and excess acrylic acid (AA) or acetoacetylethyl methacrylate (AAEM) dissolved in ethanol to the reaction after about 10% conversion of styrene, that is, well after the particle nucleation stage was complete. Individual microspheres contain ca. 10(6)-10(8) chelated lanthanide ions, of either a single element or a mixture of elements. These microspheres were characterized one-by-one utilizing a novel mass cytometer with an inductively coupled plasma (ICP) ionization source and time-of-flight (TOF) mass spectrometry detection. Microspheres containing a range of different metals at different levels of concentration were synthesized to meet the requirements of binary encoding and enumeration encoding protocols. With four different metals at five levels of concentration, we could achieve a variability of 624, and the strategy we report should allow one to obtain much larger variability. To demonstrate the usefulness of element-encoded beads for highly multiplexed immunoassays, we carried out a proof-of-principle model bioassay involving conjugation of mouse IgG to the surface of La and Tm containing particles and its detection by an antimouse IgG bearing a metal-chelating polymer with Pr.
dc.description.statementofresponsibilityAhmed I. Abdelrahman, Sheng Dai, Stuart C. Thickett, Olga Ornatsky, Dmitry Bandura, Vladimir Baranov and Mitchell A. Winnik
dc.identifier.citationJournal of the American Chemical Society, 2009; 131(42):15276-15283
dc.identifier.doi10.1021/ja9052009
dc.identifier.issn0002-7863
dc.identifier.issn1520-5126
dc.identifier.urihttp://hdl.handle.net/2440/86105
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.rights© 2009 American Chemical Society
dc.source.urihttps://doi.org/10.1021/ja9052009
dc.subjectAnimals
dc.subjectMice
dc.subjectLanthanoid Series Elements
dc.subjectPolystyrenes
dc.subjectImmunoglobulin G
dc.subjectChelating Agents
dc.subjectMicroscopy, Electron, Scanning
dc.subjectImmunoassay
dc.subjectMicrospheres
dc.subjectMolecular Structure
dc.subjectSurface Properties
dc.titleLanthanide-containing polymer microspheres by multiple-stage dispersion polymerization for highly multiplexed bioassays
dc.typeJournal article
pubs.publication-statusPublished

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