Physiological and molecular responses of pearl millet seedling to atrazine stress
Date
2018
Authors
Erinle, K.O.
Jiang, Z.
Ma, B.
Ur-Rehman, K.
Shahla, A.
Zhang, Y.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
International Journal of Phytoremediation, 2018; 20(4):343-351
Statement of Responsibility
Kehinde O. Erinle, Zhao Jiang, Bingbing Ma, Khalil Ur-Rehman, Andleeb Shahla, and Ying Zhang
Conference Name
Abstract
Pearl millet has been recommended beneficial for several therapeutic purposes. However, little is known of the physiological responses to abiotic stressors, especially of atrazine. In order to elucidate the physiological and molecular responses of pearl millet to atrazine stress, we studied the response of various biomarkers under increasing herbicide concentrations (0, 5, 10, and 50 mg/kg). We also quantified the levels of malondialdehyde (MDA) and reactive oxygen species (ROS) (H₂O₂ and O₂•-) produced in the leaves to evaluate the extent of oxidative damage. Increasing atrazine concentrations significantly increased ROS and MDA production in the plant leaves. Ascorbate peroxidase (APX) and peroxidase (POD) activities increased, while catalase (CAT) and superoxide dismutase activities reduced with increasing atrazine concentrations. Generally, atrazine applied at 50 mg/kg suppressed chlorophyll contents, whereas, chlorophyll (a/b) ratio was increased. Atrazine applied at 50 mg/kg significantly suppressed antioxidant gene expressions to the lowest. The APX gene showed overall low response to the atrazine treatments. The chloroplastic psbA gene showed highest expression with 10 mg/kg atrazine, whereas atrazine at 50 mg/kg significantly suppressed the gene expression to its lowest. Pearl millet was able to suppress oxidative stress under low atrazine levels, but high atrazine concentration could induce more oxidative damage.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
© 2018 Taylor & Francis Group, LLC