De novo reverse transcription of HTLV-1 following cell-to-cell transmission of infection

Simple item page
dc.contributor.authorBenovic, S.
dc.contributor.authorKok, T.
dc.contributor.authorStephenson, A.
dc.contributor.authorMcInnes, J.
dc.contributor.authorBurrell, C.
dc.contributor.authorLi, P.
dc.date.issued1998
dc.description.abstractAnalogous to transmission of human T-cell leukemia virus type 1 (HTLV-1) in vivo, an in vitro cell-to-cell infection model was established by coculturing MT-2 cells as virus donors and HUT78 cells as recipients. At a donor:recipient ratio of 1:2, cell fusion occurred and a new round of HTLV-1 genome replication was initiated in the cocultured cells. Newly synthesized unintegrated viral DNA was detected by Southern blot within 4-8 h and then increased between 8 and 48 h following cell mixing. The most dominant species of unintegrated viral DNA was 3.7 kb in size which hybridized to a full-length HTLV-1 DNA probe but not to a Kpnl viral DNA fragment that is absent from a defective proviral genome that has been previously identified in MT-2 cells. Northern blot analysis showed large amounts of viral RNA in the virus donor cells and in the cocultured cells, with a 3.4-kb species being the most abundant. This 3.4-kb RNA gave a pattern identical to that of the 3.7-kb unintegrated viral DNA in hybridization studies using the two probes. It seems likely that the unspliced RNA transcript from the defective proviral genome in MT-2 cells was effectively reverse transcribed upon initiation of cell-to-cell viral transmission to susceptible HUT78 cells. Despite active de novo reverse transcription, however, viral RNA levels remained unchanged following cell-to-cell transmission of HTLV-1 infection and no viral antigen production could be attributed to the newly initiated round of viral genome replication. As an abortive infection model this simple cell-to-cell infection system warrants more detailed study as it has the potential to provide reliable information regarding the early events in HTLV-1 transmission and infection.
dc.description.statementofresponsibilityBenovic, Suzana; Kok, Tuckweng; Stephenson, Alice; McInnes, James; Burrell, Christopher; Li, Peng
dc.identifier.citationVirology, 1998; 244(2):294-301
dc.identifier.doi10.1006/viro.1998.9111
dc.identifier.issn0042-6822
dc.identifier.issn1089-862X
dc.identifier.orcidBurrell, C. [0000-0002-4020-349X]
dc.identifier.urihttp://hdl.handle.net/2440/11691
dc.language.isoen
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE
dc.source.urihttps://doi.org/10.1006/viro.1998.9111
dc.subjectCell Line
dc.subjectHumans
dc.subjectHuman T-lymphotropic virus 1
dc.subjectHTLV-I Infections
dc.subjectDNA, Viral
dc.subjectRNA, Viral
dc.subjectCoculture Techniques
dc.subjectVirus Replication
dc.subjectTranscription, Genetic
dc.subjectGenome, Viral
dc.subjectModels, Biological
dc.titleDe novo reverse transcription of HTLV-1 following cell-to-cell transmission of infection
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Microbiology and Immunology publications