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Sorted cell microarrays as platforms for high-content informational bioassays

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dc.contributor.authorAnglin, E.J.
dc.contributor.authorSalisbury, C.
dc.contributor.authorBailey, S.
dc.contributor.authorHor, M.
dc.contributor.authorMacardle, P.
dc.contributor.authorFenech, M.
dc.contributor.authorThissen, H.
dc.contributor.authorVoelcker, N.H.
dc.date.issued2010
dc.description.abstractWe report on surface-engineered microarrays that provide in situ cell sorting, localization, and immobilization of various subsets of human primary lymphocytes, followed by an on-chip bioassay for ionizing-radiation-induced cytogenetic damage. The microarray format eliminates the necessity of separating cell sub-populations by alternative means (such as fluorescence- or magnetic-activated cell sorting) prior to performing informational bioassays. To exemplify the potential of this on-chip cytometry approach, we have integrated the cytokinesis-block micronucleus cytome (CBMNcyt) assay with the microarray platform for analysis of the chromosome damage profile of specific subsets of human peripheral lymphocytes. Microarray results were compared with data obtained from the traditional CBMNcyt assay on heterogeneous lymphocyte populations, and with flow cytometry data. Our results suggest that cytogenetic damage caused by ionizing radiation is not uniformly distributed across all lymphocytes subsets, but rather concentrated in specific subsets. The salient features of our approach are that it requires very small volumes of reagents, allows sorting of lymphocyte subsets in situ, increases parallelism of cell assays and is amenable to high content microscopy analysis. The onchip cytometry format opens new vistas for advanced cell-based assays, potentially bringing to light important information which remains hidden with conventional assays and hence engendering new discoveries in cell biology.
dc.identifier.citationLab on a Chip, 2010; 10(24):3413-3421
dc.identifier.doi10.1039/c0lc00185f
dc.identifier.issn1473-0197
dc.identifier.issn1473-0189
dc.identifier.orcidFenech, M. [0000-0002-8466-0991]
dc.identifier.urihttps://hdl.handle.net/1959.8/122832
dc.language.isoen
dc.publisherRoyal Society of Chemistry
dc.relation.fundingCSIRO Flagship Collaboration Fund
dc.rightsCopyright 2010 Royal Society of Chemistry
dc.source.urihttps://doi.org/10.1039/c0lc00185f
dc.subjectbioassay
dc.subjectfluorescence activated cell sorting
dc.subjectmagnetic activated cell sorting
dc.subjectmesenchymal stromal cells
dc.subjectmicroarrays
dc.subjectstem cells
dc.subjectLymphocytes
dc.subjectHumans
dc.subjectDNA Damage
dc.subjectAntibodies
dc.subjectBiological Assay
dc.subjectMicroarray Analysis
dc.subjectFlow Cytometry
dc.subjectCell Separation
dc.subjectModels, Statistical
dc.subjectMicronucleus Tests
dc.subjectDose-Response Relationship, Radiation
dc.subjectCytokinesis
dc.subjectMitosis
dc.subjectRadiation, Ionizing
dc.subjectCell Biology
dc.titleSorted cell microarrays as platforms for high-content informational bioassays
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9915910595501831

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