Rapid identification of enterovirulent Escherichia coli strains using polymerase chain reaction from shrimp farms
Date
2013
Authors
Roy, D.
Biswas, B.
Islam, H.M.R.
Ahmed, M.S.
Rasheduzzaman, M.
Sarower, M.G.
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Pakistan Journal of Biological Sciences, 2013; 16(21):1260-1269
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Abstract
Although, Escherichia coli is widely distributed in the environment, only a small percentage is pathogenic to hwnans. The most commonly encmmtered are those belonging to the Enterotoxigenic (ETEC), Enteroinvasive (EIEC), Enterohaemorrhagic (EHEC) and Enteropathogenic (EPEC) subtypes. Aquaculture premises specially shrimp farm in tropical and subtropical cormtries largely susceptive to different types of E. coli strains. With the PCR system, an attempt was taken to identify the virulent E. coli in a rapid basis from water, sediment and live shrimp from different shrimp farms established in the shrimp production areas of southwest part of Bangladesh. The target genes chosen for this investigation included the PhoA, a housekeeping gene in all E. coli and thereafter the virulent genes LT1, LT1 and ST1 of ETEC, the VI of EHEC and EAE of EPEC, which were amplified with the primers designed for their specific genes. The restriction enzyme conformation and the gel electrophoresis bands showed the presence of E. coli, among which ETEC and EPEC groups were present in the environmental and biological samples of shrimp farms, brings up into the hlllllan health concern. The sanitation conditions amid farm were also investigated to find the link of pathogenic E. coli, which came into the result of less infection if the farm maintains improved sanitation. This study has clearly urged the exigency of periodical quick check of virulent E. coli with the versatile PCR system from brood management to post-harvest handling of shrimp.
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Copyright 2013 Asian Network for Scientific Information