A rapid apoptosis assay measuring relative acridine orange fluorescence in zebrafish embryos

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dc.contributor.authorTucker, B.
dc.contributor.authorLardelli, M.
dc.contributor.organisationCentre for the Molecular Genetics of Development
dc.date.issued2007
dc.descriptionCopyright © 2007 Mary Ann Liebert, Inc.
dc.description.abstractThe ability to easily analyze apoptosis is important in studies of molecular cell biology and to evaluate the relative toxicity of different treatments or environments. This is particularly the case when substances such as morpholino oligonucleotides are injected into embryos, as such treatments can cause widespread, complex patterns of apoptosis. Zebrafish embryos are well suited for cell biological and environmental toxicity analyses, but the need remains for a simple method that can analyze levels of apoptosis in a statistically significant number of embryos. Here we present a "group fluorescence" method for rapid, large-scale analysis of relative levels of apoptosis based on densitometric techniques.
dc.description.statementofresponsibilityBen Tucker and Michael Lardelli
dc.identifier.citationZebrafish, 2007; 4(2):113-116
dc.identifier.doi10.1089/zeb.2007.0508
dc.identifier.issn1545-8547
dc.identifier.issn1557-8542
dc.identifier.orcidLardelli, M. [0000-0002-4289-444X]
dc.identifier.urihttp://hdl.handle.net/2440/43663
dc.language.isoen
dc.publisherMary Ann Liebert, Inc. Publishers
dc.source.urihttps://doi.org/10.1089/zeb.2007.0508
dc.subjectEmbryo, Nonmammalian
dc.subjectAnimals
dc.subjectZebrafish
dc.subjectAcridine Orange
dc.subjectOligonucleotides, Antisense
dc.subjectFluorescent Dyes
dc.subjectDensitometry
dc.subjectStaining and Labeling
dc.subjectApoptosis
dc.subjectFluorescence
dc.titleA rapid apoptosis assay measuring relative acridine orange fluorescence in zebrafish embryos
dc.typeJournal article
pubs.publication-statusPublished

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