Inhibition of the Pim1 oncogene results in diminished visual function

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dc.contributor.authorYin, J.
dc.contributor.authorShine, L.
dc.contributor.authorRaycroft, F.
dc.contributor.authorDeeti, S.
dc.contributor.authorReynolds, A.
dc.contributor.authorAckerman, K.
dc.contributor.authorGlaviano, A.
dc.contributor.authorO'Farrell, S.
dc.contributor.authorO'Leary, O.
dc.contributor.authorKilty, C.
dc.contributor.authorKennedy, C.
dc.contributor.authorMcLoughlin, S.
dc.contributor.authorRice, M.
dc.contributor.authorRussell, E.
dc.contributor.authorHiggins, D.
dc.contributor.authorHyde, D.
dc.contributor.authorKennedy, B.
dc.contributor.editorThummel, R.
dc.date.issued2012
dc.description.abstractOur objective was to profile genetic pathways whose differential expression correlates with maturation of visual function in zebrafish. Bioinformatic analysis of transcriptomic data revealed Jak-Stat signalling as the pathway most enriched in the eye, as visual function develops. Real-time PCR, western blotting, immunohistochemistry and in situ hybridization data confirm that multiple Jak-Stat pathway genes are up-regulated in the zebrafish eye between 3-5 days post-fertilisation, times associated with significant maturation of vision. One of the most up-regulated Jak-Stat genes is the proto-oncogene Pim1 kinase, previously associated with haematological malignancies and cancer. Loss of function experiments using Pim1 morpholinos or Pim1 inhibitors result in significant diminishment of visual behaviour and function. In summary, we have identified that enhanced expression of Jak-Stat pathway genes correlates with maturation of visual function and that the Pim1 oncogene is required for normal visual function.
dc.description.statementofresponsibilityJun Yin, Lisa Shine, Francis Raycroft, Sudhakar Deeti, Alison Reynolds, Kristin M. Ackerman, Antonino Glaviano, Sean O'Farrell, Olivia O'Leary, Claire Kilty, Ciaran Kennedy, Sarah McLoughlin, Megan Rice, Eileen Russell, Desmond G. Higgins, David R. Hyde, Breandan N. Kennedy
dc.identifier.citationPLoS One, 2012; 7(12):e52177-1-e52177-25
dc.identifier.doi10.1371/journal.pone.0052177
dc.identifier.issn1932-6203
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/2440/90004
dc.language.isoen
dc.publisherPublic Library of Science
dc.rights© 2012 Yin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.source.urihttps://doi.org/10.1371/journal.pone.0052177
dc.subjectAnimals
dc.titleInhibition of the Pim1 oncogene results in diminished visual function
dc.typeJournal article
pubs.publication-statusPublished

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