Demonstration of the lack of cytotoxicity of unmodified and folic acid modified graphene oxide quantum dots, and their application to fluorescence lifetime imaging of HaCaT cells
| dc.contributor.author | Goreham, R.V. | |
| dc.contributor.author | Schroeder, K.L. | |
| dc.contributor.author | Holmes, A. | |
| dc.contributor.author | Bradley, S.J. | |
| dc.contributor.author | Nann, T. | |
| dc.date.issued | 2018 | |
| dc.description | Data source: Supplementary material, https://doi.org/10.1007/s00604-018-2679-8 | |
| dc.description.abstract | The authors describe the synthesis of water-soluble and fluorescent graphene oxide quantum dots via acid exfoliation of graphite nanoparticles. The resultant graphene oxide quantum dots (GoQDs) were then modified with folic acid. Folic acid receptors are overexpressed in cancer cells and hence can bind to functionalized graphene oxide quantum dots. On excitation at 305 nm, the GoQDs display green fluorescence with a peak wavelength at ~520 nm. The modified GoQDs are non-toxic to macrophage cells even after prolonged exposure and high concentrations. Fluorescence lifetime imaging and multiphoton microscopy was used (in combination) to image HeCaT cells exposed to GoQDs, resulting in a superior method for bioimaging. | |
| dc.identifier.citation | Microchimica Acta, 2018; 185(2):128-134 | |
| dc.identifier.doi | 10.1007/s00604-018-2679-8 | |
| dc.identifier.issn | 0026-3672 | |
| dc.identifier.issn | 1436-5073 | |
| dc.identifier.uri | https://hdl.handle.net/11541.2/130503 | |
| dc.language.iso | en | |
| dc.publisher | Springer | |
| dc.rights | Copyright 2018 Springer-Verlag GmbH Austria, part of Springer Nature | |
| dc.source.uri | https://doi.org/10.1007/s00604-018-2679-8 | |
| dc.subject | toxicity | |
| dc.subject | .LDH assay | |
| dc.subject | alamar blue assay | |
| dc.subject | graphene quantum dots | |
| dc.subject | bioimaging | |
| dc.subject | fluorescence lifetime | |
| dc.subject | multiphoton microscopy | |
| dc.title | Demonstration of the lack of cytotoxicity of unmodified and folic acid modified graphene oxide quantum dots, and their application to fluorescence lifetime imaging of HaCaT cells | |
| dc.type | Journal article | |
| pubs.publication-status | Published | |
| ror.mmsid | 9916174111701831 |