Contrasting roles for RANTES and macrophage inflammatory protein-1α (MIP-1α) in a murine model of allergic peritonitis

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dc.contributor.authorDas, A.
dc.contributor.authorAjuebor, M.
dc.contributor.authorFlower, R.
dc.contributor.authorPerretti, M.
dc.contributor.authorMcColl, S.
dc.date.issued1999
dc.description.abstractCell accumulation and CC chemokine production were assessed in the peritoneal cavity of ovalbumin (OVA)-sensitized mice following antigen challenge. Intraperitoneal challenge with OVA induced a significant eosinophil influx from 6 h post-challenge with increased numbers persisting at 24 h. At 6 h there was also a marked presence of neutrophils. Messenger RNA expression and protein levels for the chemokines RANTES and MIP-1 alpha were measured in the cell pellets and supernatants, respectively, from peritoneal washes following OVA challenge. RANTES mRNA was detected from 2 h to 4 h following OVA injection, whereas mRNA for MIP-1 alpha was only detectable at 4 h. RANTES protein was first detected from 4 h after OVA injection and by 24 h the protein levels had increased further. Basal levels of MIP-1 alpha were detected in peritoneal washes. These levels peaked at 2 h after OVA challenge and rapidly declined to basal levels by 6 h. A functional role for the chemokines was assessed using neutralizing polyclonal antibodies. Co-injection of OVA with anti-RANTES antibodies resulted in a significant inhibition of eosinophil infiltration into the cavity at 6 h and 24 h (63% and 52% inhibition, respectively) without significantly influencing the number of neutrophils present. In contrast, injection of anti-MIP-1 alpha antibodies only inhibited neutrophil migration at the 6 h time point by 44% without significantly affecting the accumulation of eosinophils. These results demonstrate an important role for RANTES in mediating eosinophil influx in allergic inflammation and a contrasting role for MIP-1 alpha in mediating neutrophil recruitment.
dc.identifier.citationClinical and Experimental Immunology, 1999; 117(2):223-229
dc.identifier.doi10.1046/j.1365-2249.1999.00978.x
dc.identifier.issn0009-9104
dc.identifier.issn1365-2249
dc.identifier.orcidMcColl, S. [0000-0003-0949-4660]
dc.identifier.urihttp://hdl.handle.net/2440/11548
dc.language.isoen
dc.publisherWILEY
dc.source.urihttps://doi.org/10.1046/j.1365-2249.1999.00978.x
dc.subjectPeritoneal Cavity
dc.subjectEosinophils
dc.subjectNeutrophils
dc.subjectAnimals
dc.subjectMice, Inbred BALB C
dc.subjectMice
dc.subjectPeritonitis
dc.subjectHypersensitivity
dc.subjectDisease Models, Animal
dc.subjectOvalbumin
dc.subjectMacrophage Inflammatory Proteins
dc.subjectImmune Sera
dc.subjectInjections, Intraperitoneal
dc.subjectCell Movement
dc.subjectTime Factors
dc.subjectFemale
dc.subjectChemokine CCL5
dc.subjectChemokine CCL4
dc.titleContrasting roles for RANTES and macrophage inflammatory protein-1α (MIP-1α) in a murine model of allergic peritonitis
dc.title.alternativeContrasting roles for RANTES and macrophage inflammatory protein-1 alpha (MIP-1 alpha) in a murine model of allergic peritonitis
dc.typeJournal article
pubs.publication-statusPublished

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