A rapid method for the screening of fatty acids in lipids in plasma or serum without prior extraction

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dc.contributor.authorLiu, G.
dc.contributor.authorMakrides, M.
dc.contributor.authorCoates, P.
dc.contributor.authorLam, K.
dc.contributor.authorRanieri, E.
dc.contributor.authorMas, E.
dc.contributor.authorGibson, R.A.
dc.date.issued2022
dc.description.abstractSerum or plasma are the commonly used blood fractions to determine the relationship between dietary and circulating fatty acids in health and disease. Most methods available for the measurement of fatty acids in serum or plasma (referred to as serum henceforth) require prior extraction with organic solvents. We have determined that it is possible to directly convert the lipids in aqueous biological samples to fatty acid methyl esters (FAME) without prior extraction, providing that the ratio of serum to transmethylation solvent does not exceed 10%. Our in-vial transmethylation system uses 50uL serum pipetted into 2 mL screw top GC vials containing 1 mL of 1% H2SO4 in methanol at 50 ◦C and subsequent FAME extracted in the same vial into 300uL heptane. The system yields both compositional and quantitative analysis of the fatty acids of serum identical to conventional standard methods. Evaluation of our new serum assay confirms significant correlations between the fatty acid measures and those obtained from conventional standard assay for all fatty acids (r > 0.99, P<0.0001), including the n-6 (r = 0.998, P<0.0001) and n-3 long chain polyunsaturated fatty acids (r = 0.993, P<0.0001). There were high levels of agreement between methods on Bland -Altman analysis, indicating the interchangeability of the methods. These results establish our new method as reliable for the assessment of fatty acid composition of small volumes of serum useful for high throughput situations that limits the volume of organic solvents and technical input.
dc.description.statementofresponsibilityGe Liu, Maria Makrides, Penelope Coates, Khoa Lam, Enzo Ranieri, Emilie Mas, Robert A Gibson
dc.identifier.citationProstaglandins Leukotrienes and Essential Fatty Acids, 2022; 178:102416-1-102416-7
dc.identifier.doi10.1016/j.plefa.2022.102416
dc.identifier.issn0952-3278
dc.identifier.issn1532-2823
dc.identifier.orcidMakrides, M. [0000-0003-3832-541X]
dc.identifier.orcidMas, E. [0000-0003-2848-9613]
dc.identifier.orcidGibson, R.A. [0000-0002-8750-525X]
dc.identifier.urihttps://hdl.handle.net/2440/135188
dc.language.isoen
dc.publisherElsevier
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1135155
dc.rights© 2022 Elsevier Ltd. All rights reserved
dc.source.urihttps://doi.org/10.1016/j.plefa.2022.102416
dc.subjectPlasma
dc.subjectSerum
dc.subjectn-3 fatty acids
dc.subjectDocosahexaenoic acid
dc.subjectHigh throughput
dc.subjectFAME
dc.subject.meshFatty Acids, Omega-3
dc.subject.meshFatty Acids
dc.subject.meshSolvents
dc.titleA rapid method for the screening of fatty acids in lipids in plasma or serum without prior extraction
dc.typeJournal article
pubs.publication-statusPublished

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