Sequences of the genomic DNAs encoding the S2, S9, S10, and S23 alleles from almond, Prunus dulcis
Date
2002
Authors
Channuntapipat, Chockpisit
Sedgley, Margaret
Batlle, Ignasi
Arus, Pere
Collins, Graham G.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Journal of Horticultural Science and Biotechnology, 2002; 77(4):387-392
Statement of Responsibility
Chockpisit Channuntapipat, Margaret Sedgle, Ignasi Batlle, Pere Arús And Graham Collins
Conference Name
Abstract
Partial genomic sequences of the S-alleles S2, S9, and S10, identified in genomic DNA selected from Prunus dulcis ´Cristomorto` (S1S2), ´Anxaneta` (S2S9), ´Primorskiy` (S5S9), and ´Gabaix` (S5S10), were found to contain introns of 562, 1,343, and ∼705 bp, and partial exons of 537, 486, and 495 bp respectively. In addition, another allele, characterized as S23, was identified in the two Australian cultivars Johnston`s Prolific and Pierce. This allele had an intron size of 662 bp and a partial exon size of 489 bp, and may have been introduced into Australia in the Spanish cultivar Ramillete (S6S23). The exoni/ntron splice junction sites of all alleles followed the GT/AG consensus sequence rule, and the sequences were found to be highly conserved.