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Sn1,3 regiospecificity of DHA (22:6ω-3) of plant origin (DHA-Canola<sup>®</sup>) facilitates its preferential tissue incorporation in rats compared to sn2 DHA in algal oil at low dietary inclusion levels

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dc.contributor.authorBelobrajdic, D.P.
dc.contributor.authorDallimore, J.A.
dc.contributor.authorAdams, M.J.
dc.contributor.authorSingh, S.P.
dc.contributor.authorAbeywardena, M.Y.
dc.date.issued2025
dc.descriptionData source: Supplementary materials, https://www.mdpi.com/article/10.3390/nu17081306/s1
dc.description.abstractBackground/Objectives: Regiospecificity in triacylglycerols (TAGs) influences absorption/bioavailability of dietary fatty acids. We evaluated whether sn1,3 located DHA (22:6ω3) of a transgenic higher plant (DHA-Canola®) preferentially facilitates its tissue incorporation as compared to sn2 positioned DHA (DHASCO® of algal origin). Methods: Sprague Dawley rats were fed diets (12 weeks) containing DHA-Canola or DHA-Control (a blend of DHASCO® and high oleic sunflower seed oil (HOSO)) at 0.3%, 1%, 3%, and 6% (w/w), or 7% HOSO prior to determination of tissue fatty acids. Results: At 0.3 and 1% w/w supplementation, plasma, liver and cardiac tissue DHA incorporation was higher in the plant-based oil (DHA-Canola vs. DHA-Control; p < 0.05), whilst sn2 enriched algal oil yielded better outcomes at higher doses (at 3% inclusion, plasma values were 7.8 vs. 5.9%, and at 6% supplementation, 10.0 vs. 7.9 in favor of DHA-Control, p < 0.05) At lower intakes, sn1,3 regiospecificity (DHA-Canola) increased the omega-3 index, a clinically relevant biomarker, compared to DHA-Control (p < 0.05). Similarly, a build-up of 20:5ω3 and 22:5ω3 occurred with DHA-Canola. Consequently, total omega3s were higher in this latter group. Conclusions: At lower intakes, sn1,3 regiospecificity of DHA leads to its preferential tissue incorporation compared to sn2 DHA.
dc.identifier.citationNutrients, 2025; 17(8):1-18
dc.identifier.doi10.3390/nu17081306
dc.identifier.issn2072-6643
dc.identifier.issn2072-6643
dc.identifier.orcidBelobrajdic, D.P. [0000-0002-5801-8663]
dc.identifier.urihttps://hdl.handle.net/11541.2/43028
dc.language.isoen
dc.publisherMDPI AG
dc.relation.fundingNuSeed Australia
dc.rightsCopyright 2025 by the authors.Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. (https://creativecommons.org/licenses/by/4.0/)
dc.source.urihttps://doi.org/10.3390/nu17081306
dc.subjectcanola
dc.subjectDHA
dc.subjectDPA
dc.subjectEPA
dc.subjectfish oil
dc.subjectKrill oil
dc.subjectomega-3 index
dc.subjectω-3 fatty acids
dc.subjectω-3 LCPUFA
dc.titleSn1,3 regiospecificity of DHA (22:6ω-3) of plant origin (DHA-Canola<sup>®</sup>) facilitates its preferential tissue incorporation in rats compared to sn2 DHA in algal oil at low dietary inclusion levels
dc.typeJournal article
pubs.publication-statusPublished
ror.fileinfo12301970660001831 13301990780001831 Open Access Published Version
ror.mmsid9916966874801831

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