Effect of epidermal growth factor-like peptides on the metabolism of in vitro matured mouse oocytes and cumulus cells

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dc.contributor.authorRichani, D.
dc.contributor.authorSutton-McDowall, M.
dc.contributor.authorFrank, L.
dc.contributor.authorGilchrist, R.
dc.contributor.authorThompson, J.
dc.contributor.organisationRobinson Institute
dc.date.issued2014
dc.description.abstractOocyte in vitro maturation (IVM) is an assisted reproductive technology that involves the maturation of cumulus-oocyte-complexes (COCs) that are then capable of normal development. We have shown that epidermal growth factor (EGF)-like peptide signalling is perturbed in mouse COCs undergoing IVM when matured with FSH and/or EGF, but supplementation of IVM with EGF-like peptides amphiregulin or epiregulin improves oocyte developmental competence. Here we aimed to determine whether EGF-like peptides regulate COC metabolism. Immature 129/Sv mouse COCs underwent IVM with FSH, EGF, amphiregulin, epiregulin, betacellulin, or no treatment (control). Epiregulin significantly increased intra-oocyte FAD++ and REDOX ratio compared to FSH and control. Amphiregulin and epiregulin significantly increased the proportion of J-aggregates (from JC-1) in oocyte mitochondria compared to control, FSH or EGF, and this coupled with FAD++ and REDOX measures indicates greater mitochondrial activity. There were no differences in glucose consumption, lactate production, or glycolysis between COCs matured with FSH, EGF and EGF-like peptides. COCs matured with EGF or EGF-like peptides exhibited significantly higher mRNA expression of the hexosamine biosynthesis pathway (HBP) rate-limiting enzyme gene Gfpt2, Has2 expression, and global beta-O-linked glycosylation of proteins, compared to control or FSH, suggesting greater HBP activity. Our findings suggest that; (i) EGF-like peptides, particularly epiregulin, induce more oocyte mitochondrial activity than EGF or FSH, and (ii) EGF-like peptides and EGF induce greater HBP activity, enabling more hyaluronic acid synthesis and protein beta-O-linked glycosylation. These metabolic alterations may be a mechanism by which EGF-like peptides increase oocyte developmental competence.
dc.description.statementofresponsibilityDulama Richani, Melanie L. Sutton-McDowall, Laura A. Frank, Robert B. Gilchrist, and Jeremy G. Thompson
dc.identifier.citationBiology of Reproduction, 2014; 90(3):49-49
dc.identifier.doi10.1095/biolreprod.113.115311
dc.identifier.issn0006-3363
dc.identifier.issn1529-7268
dc.identifier.orcidSutton-McDowall, M. [0000-0002-4121-0202]
dc.identifier.orcidGilchrist, R. [0000-0003-1611-7142]
dc.identifier.orcidThompson, J. [0000-0003-4941-7731]
dc.identifier.urihttp://hdl.handle.net/2440/81927
dc.language.isoen
dc.publisherSociety for the Study of Reproduction
dc.rightsCopyright 2014 by The Society for the Study of Reproduction.
dc.source.urihttps://doi.org/10.1095/biolreprod.113.115311
dc.subjectCumulus cells
dc.subjectGrowth factors
dc.subjectIn vitro maturation (IVM)
dc.subjectMetabolism
dc.subjectOocyte maturation
dc.titleEffect of epidermal growth factor-like peptides on the metabolism of in vitro matured mouse oocytes and cumulus cells
dc.typeJournal article
pubs.publication-statusPublished

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