Current studentsExisting staffContact us
 

Development of wheat-Aegilops speltoides recombinants and simple PCR-based markers for Sr32 and a new stem rust resistance gene on the 2S#1 chromosome

Simple item page
dc.contributor.authorMago, R.
dc.contributor.authorVerlin, D.
dc.contributor.authorZhang, P.
dc.contributor.authorBansal, U.
dc.contributor.authorBariana, H.
dc.contributor.authorJin, Y.
dc.contributor.authorEllis, J.
dc.contributor.authorHoxha, S.
dc.contributor.authorDundas, I.
dc.date.issued2013
dc.description.abstractWild relatives of wheat are important resources for new rust resistance genes but underutilized because the valuable resistances are often linked to negative traits that prevent deployment of these genes in commercial wheats. Here, we report ph1b-induced recombinants with reduced alien chromatin derived from E.R. Sears’ wheat–Aegilops speltoides 2D-2S#1 translocation line C82.2, which carries the widely effective stem rust resistance gene Sr32. Infection type assessments of the recombinants showed that the original translocation in fact carries two stem rust resistance genes, Sr32 on the short arm and a previously undescribed gene SrAes1t on the long arm of chromosome 2S#1. Recombinants with substantially shortened alien chromatin were produced for both genes, which confer resistance to stem rust races in the TTKSK (Ug99) lineage and representative races of all Australian stem rust lineages. Selected recombinants were back crossed into adapted Australian cultivars and PCR markers were developed to facilitate the incorporation of these genes into future wheat varieties. Our recombinants and those from several other labs now show that Sr32, Sr39, and SrAes7t on the short arm and Sr47 and SrAes1t on the long arm of 2S#1 form two linkage groups and at present no rust races are described that can distinguish these resistance specificities.
dc.description.statementofresponsibilityRohit Mago, Dawn Verlin, Peng Zhang, Urmil Bansal, Harbans Bariana, Yue Jin, Jeffrey Ellis, Sami Hoxha, Ian Dundas
dc.identifier.citationTheoretical and Applied Genetics: international journal of plant breeding research, 2013; 126(12):2943-2955
dc.identifier.doi10.1007/s00122-013-2184-8
dc.identifier.issn0040-5752
dc.identifier.issn1432-2242
dc.identifier.urihttp://hdl.handle.net/2440/87140
dc.language.isoen
dc.publisherSpringer-Verlag
dc.rights© Springer-Verlag Berlin Heidelberg 2013
dc.source.urihttps://doi.org/10.1007/s00122-013-2184-8
dc.subjectChromosomes, Plant
dc.subjectChromatin
dc.subjectBasidiomycota
dc.subjectTriticum
dc.subjectPlant Stems
dc.subjectGenetic Markers
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectPolymerase Chain Reaction
dc.subjectPlant Diseases
dc.subjectGenes, Plant
dc.subjectHomologous Recombination
dc.titleDevelopment of wheat-Aegilops speltoides recombinants and simple PCR-based markers for Sr32 and a new stem rust resistance gene on the 2S#1 chromosome
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Agriculture, Food and Wine publications