-308 Nco I polymorphism of tumour necrosis factor a in overweight caucasians

Simple item page
dc.contributor.authorCoates, A.
dc.contributor.authorHeilbronn, L.
dc.contributor.authorNoakes, M.
dc.contributor.authorKind, K.
dc.contributor.authorClifton, P.
dc.date.issued2003
dc.description.abstractWe investigated whether a polymorphism in the promoter region of the TNFα gene (−308 A/G) is associated with reduced weight loss in obese Australian subjects on an energy restricted diet. 189 healthy subjects and 91 subjects with type II diabetes were genotyped for the −308 Nco I polymorphism using PCR-RFLP techniques. A subset of these subjects (211 females and 45 males), were placed on a 30% energy restricted diet (6200 kJ) for 12 weeks. Subjects were assessed every 2 weeks and changes in body weight, waist circumference and BMI were used as determinants of weight loss. Fasting plasma was analysed for glucose, insulin, lipids and free fatty acids. 64% of subjects were GG homozygotes, 31% were AG heterozygotes and 5% were AA homozygotes. There was no significant difference between the allele frequency in healthy subjects (0.21) and type 2 diabetic patients (0.24). The presence of the −308 A/G polymorphism did not significantly influence initial BMI, the amount of weight lost (GG, 8.1±0.65 kg, AG, 6.9±0.77 kg, AA, 7.6±0.12 kg), waist circumference or any metabolic variable. The AA variant at position −308 in the promoter region of the TNFα gene does not influence the amount of weight lost in overweight and obese men and women on a 30% energy restricted diet.
dc.description.statementofresponsibilityAlison M. Morris, Leonie K. Heilbronn, Manny Noakes, Karen L. Kind and Peter M. Clifton
dc.description.urihttp://www.elsevier.com/wps/find/journaldescription.cws_home/505949/description#description
dc.identifier.citationDiabetes Research and Clinical Practice, 2003; 62(3):197-201
dc.identifier.doi10.1016/j.diabres.2003.08.002
dc.identifier.issn0168-8227
dc.identifier.issn1872-8227
dc.identifier.orcidHeilbronn, L. [0000-0003-2106-7303]
dc.identifier.orcidClifton, P. [0000-0002-6411-626X]
dc.identifier.urihttp://hdl.handle.net/2440/2972
dc.language.isoen
dc.publisherElsevier Sci Ireland Ltd
dc.source.urihttps://doi.org/10.1016/j.diabres.2003.08.002
dc.subjectTumour necrosis factor alpha polymorphism
dc.subjectobesity
dc.subjectweight loss
dc.title-308 Nco I polymorphism of tumour necrosis factor a in overweight caucasians
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Molecular and Biomedical Science publications
Agriculture, Food and Wine publications