Purification and characterization of alkaline pectin lyase from a newly isolated Bacillus clausii and its application in elicitation of plant disease resistance

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dc.contributor.authorLi, Z.
dc.contributor.authorBai, Z.
dc.contributor.authorZhang, B.
dc.contributor.authorLi, B.
dc.contributor.authorJin, B.
dc.contributor.authorZhang, M.
dc.contributor.authorLin, F.
dc.contributor.authorZhang, H.
dc.date.issued2012
dc.description.abstractAlkaline pectin lyase (PNL) shows potential as a biological control agent against several plant diseases. We isolated and characterized a new Bacillus clausii strain that can produce 4,180 U/g of PNL using sugar beet pulp as a carbon source and inducer. The PNL was purified to apparent homogeneity using ultrafiltration, ammonium sulfate fractionation, DEAE Sepharose Fast Flow, and Sephadex G-75 gel filtration. The purified PNL was found to be a monomeric protein with a molecular weight of 35 kDa, as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). It demonstrated optimal activity with K m of 0.87 mg/ml at pH 10.0 and 60 °C. The enzyme is stable in the pH range of 8.0–10.0 and temperature ≤40 °C. Ca2+ was found to stimulate the enzymatic activity of the PNL by up to 410 %. Mass spectrometric results gave 38 % match coverage with pectate lyase from B. clausii KSM-K16 (gi|56961845). The PNL was found to elicit disease resistance in cucumber seedlings, suggesting that it may have applications in biocontrol and sustainable agriculture.
dc.description.statementofresponsibilityZuming Li, Zhihui Bai, Baoguo Zhang, Baojv Li, Bo Jin, Michael Zhang, Francis Lin and Hongxun Zhang
dc.identifier.citationApplied Biochemistry and Biotechnology, 2012; 167(8):2241-2256
dc.identifier.doi10.1007/s12010-012-9758-9
dc.identifier.issn0273-2289
dc.identifier.issn1559-0291
dc.identifier.urihttp://hdl.handle.net/2440/73757
dc.language.isoen
dc.publisherHumana Press Inc
dc.rights© Springer Science+Business Media, LLC 2012
dc.source.urihttps://doi.org/10.1007/s12010-012-9758-9
dc.subjectBacillus clausii
dc.subjectAlkaline pectin lyase
dc.subjectsugar beet pulp
dc.subjectmass spectrometric sequencing
dc.subjectplant disease resistance
dc.titlePurification and characterization of alkaline pectin lyase from a newly isolated Bacillus clausii and its application in elicitation of plant disease resistance
dc.typeJournal article
pubs.publication-statusPublished

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