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Truncating variants in NAA15 are associated with variable levels of intellectual disability, autism spectrum disorder, and congenital anomalies

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dc.contributor.authorCheng, H.
dc.contributor.authorDharmadhikari, A.
dc.contributor.authorVarland, S.
dc.contributor.authorMa, N.
dc.contributor.authorDomingo, D.
dc.contributor.authorKleyner, R.
dc.contributor.authorRope, A.
dc.contributor.authorYoon, M.
dc.contributor.authorStray-Pedersen, A.
dc.contributor.authorPosey, J.
dc.contributor.authorCrews, S.
dc.contributor.authorEldomery, M.
dc.contributor.authorAkdemir, Z.
dc.contributor.authorLewis, A.
dc.contributor.authorSutton, V.
dc.contributor.authorRosenfeld, J.
dc.contributor.authorConboy, E.
dc.contributor.authorAgre, K.
dc.contributor.authorXia, F.
dc.contributor.authorWalkiewicz, M.
dc.contributor.authoret al.
dc.date.issued2018
dc.description.abstractN-alpha-acetylation is a common co-translational protein modification that is essential for normal cell function in humans. We previously identified the genetic basis of an X-linked infantile lethal Mendelian disorder involving a c.109T>C (p.Ser37Pro) missense variant in NAA10, which encodes the catalytic subunit of the N-terminal acetyltransferase A (NatA) complex. The auxiliary subunit of the NatA complex, NAA15, is the dimeric binding partner for NAA10. Through a genotype-first approach with whole-exome or genome sequencing (WES/WGS) and targeted sequencing analysis, we identified and phenotypically characterized 38 individuals from 33 unrelated families with 25 different de novo or inherited, dominantly acting likely gene disrupting (LGD) variants in NAA15. Clinical features of affected individuals with LGD variants in NAA15 include variable levels of intellectual disability, delayed speech and motor milestones, and autism spectrum disorder. Additionally, mild craniofacial dysmorphology, congenital cardiac anomalies, and seizures are present in some subjects. RNA analysis in cell lines from two individuals showed degradation of the transcripts with LGD variants, probably as a result of nonsense-mediated decay. Functional assays in yeast confirmed a deleterious effect for two of the LGD variants in NAA15. Further supporting a mechanism of haploinsufficiency, individuals with copy-number variant (CNV) deletions involving NAA15 and surrounding genes can present with mild intellectual disability, mild dysmorphic features, motor delays, and decreased growth. We propose that defects in NatA-mediated N-terminal acetylation (NTA) lead to variable levels of neurodevelopmental disorders in humans, supporting the importance of the NatA complex in normal human development.
dc.description.statementofresponsibilityHanyin Cheng, Avinash V. Dharmadhikari, Sylvia Varland … Mark A. Corbett … Jozef Gecz … Marie A. Shaw … et al.
dc.identifier.citationAmerican Journal of Human Genetics, 2018; 102(5):985-994
dc.identifier.doi10.1016/j.ajhg.2018.03.004
dc.identifier.issn0002-9297
dc.identifier.issn1537-6605
dc.identifier.orcidCorbett, M. [0000-0001-9298-3072]
dc.identifier.orcidShaw, M. [0000-0002-5060-190X]
dc.identifier.orcidGardner, A. [0009-0009-7321-1697]
dc.identifier.orcidBarnett, C. [0000-0003-1717-3824]
dc.identifier.orcidKassahn, K. [0000-0002-1662-3355]
dc.identifier.orcidGecz, J. [0000-0002-7884-6861]
dc.identifier.urihttp://hdl.handle.net/2440/112992
dc.language.isoen
dc.publisherCell Press
dc.rights© 2018 American Society of Human Genetics.
dc.source.urihttps://doi.org/10.1016/j.ajhg.2018.03.004
dc.subjectN-terminal acetylation (NTA)
dc.subjectN-terminal acetyltransferases (NATs)
dc.subjectNAA10
dc.subjectNAA15
dc.subjectNatA complex
dc.subjectOgden syndrome
dc.subjectautism
dc.subjectcongenital heart defects
dc.subjectintellectual disability
dc.subjectneurodevelopmental disorder
dc.titleTruncating variants in NAA15 are associated with variable levels of intellectual disability, autism spectrum disorder, and congenital anomalies
dc.typeJournal article
pubs.publication-statusPublished

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