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The effect of discrete wavelengths of visible light on the developing murine embryo

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dc.contributor.authorCampugan, C.A.
dc.contributor.authorLim, M.
dc.contributor.authorChow, D.J.X.
dc.contributor.authorTan, T.C.Y.
dc.contributor.authorLi, T.
dc.contributor.authorSaini, A.A.
dc.contributor.authorOrth, A.
dc.contributor.authorReineck, P.
dc.contributor.authorSchartner, E.P.
dc.contributor.authorThompson, J.G.
dc.contributor.authorDholakia, K.
dc.contributor.authorDunning, K.R.
dc.date.issued2022
dc.descriptionThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
dc.description.abstractPurpose: A current focus of the IVF field is non-invasive imaging of the embryo to quantify developmental potential. Such approaches use varying wavelengths to gain maximum biological information. The impact of irradiating the developing embryo with discrete wavelengths of light is not fully understood. Here, we assess the impact of a range of wavelengths on the developing embryo. Methods: Murine preimplantation embryos were exposed daily to wavelengths within the blue, green, yellow, and red spectral bands and compared to an unexposed control group. Development to blastocyst, DNA damage, and cell number/allocation to blastocyst cell lineages were assessed. For the longer wavelengths (yellow and red), pregnancy/fetal outcomes and the abundance of intracellular lipid were investigated. Results: Significantly fewer embryos developed to the blastocyst stage when exposed to the yellow wavelength. Elevated DNA damage was observed within embryos exposed to blue, green, or red wavelengths. There was no effect on blastocyst cell number/lineage allocation for all wavelengths except red, where there was a significant decrease in total cell number. Pregnancy rate was significantly reduced when embryos were irradiated with the red wavelength. Weight at weaning was significantly higher when embryos were exposed to yellow or red wavelengths. Lipid abundance was significantly elevated following exposure to the yellow wavelength. Conclusion: Our results demonstrate that the impact of light is wavelength-specific, with longer wavelengths also impacting the embryo. We also show that effects are energy-dependent. This data shows that damage is multifaceted and developmental rate alone may not fully reflect the impact of light exposure.
dc.description.statementofresponsibilityCarl A. Campugan, Megan Lim, Darren J. X. Chow, Tiffany C. Y. Tan, Tong Li, Avishkar A. Saini, Antony Orth, Philipp Reineck, Erik P. Schartner, Jeremy G. Thompson, Kishan Dholakia, Kylie R. Dunning
dc.identifier.citationJournal of Assisted Reproduction and Genetics, 2022; 39(8):1825-1837
dc.identifier.doi10.1007/s10815-022-02555-4
dc.identifier.issn1058-0468
dc.identifier.issn1573-7330
dc.identifier.orcidCampugan, C.A. [0000-0003-4947-8251]
dc.identifier.orcidLim, M. [0000-0002-9868-429X]
dc.identifier.orcidChow, D.J.X. [0000-0002-2648-4600]
dc.identifier.orcidLi, T. [0000-0003-0959-2084]
dc.identifier.orcidSaini, A.A. [0000-0002-2221-7476]
dc.identifier.orcidSchartner, E.P. [0000-0003-1669-4302]
dc.identifier.orcidThompson, J.G. [0000-0003-4941-7731]
dc.identifier.orcidDholakia, K. [0000-0001-6534-9009]
dc.identifier.orcidDunning, K.R. [0000-0002-0462-6479]
dc.identifier.urihttps://hdl.handle.net/2440/135694
dc.language.isoen
dc.publisherSpringer Science and Business Media LLC
dc.relation.granthttp://purl.org/au-research/grants/arc/FL210100099
dc.relation.granthttp://purl.org/au-research/grants/arc/CE140100003
dc.relation.granthttp://purl.org/au-research/grants/arc/DE200100279
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/2003786
dc.rights© The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
dc.source.urihttps://doi.org/10.1007/s10815-022-02555-4
dc.subjectPhotodamage; Phototoxicity; Microscopy; Preimplantation embryo; Blastocyst
dc.titleThe effect of discrete wavelengths of visible light on the developing murine embryo
dc.typeJournal article
pubs.publication-statusPublished

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