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Differential effects of insulin-like growth factors on scratch wound repair in respiratory epithelial cells

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dc.contributor.authorAdams, D.
dc.contributor.authorMcIntosh, D.
dc.contributor.authorWormald, P.
dc.contributor.authorCowin, A.
dc.date.issued2006
dc.description.abstractBackground: Insulin-like growth factors (IGFs) I and II, being potent promoters of cellular growth and differentiation, were investigated for their effectiveness in improving the rate of scratch closure in human respiratory epithelium in vitro. Methods: Human epithelial cell lines from the nasal, bronchial, and tracheal regions were analyzed for their response to IGF-I and IGF-II, in a confluent monolayer scratch assay. IGF-binding proteins (IGFBPs) produced by certain cells are able to reduce the effectiveness of the IGFs. Consequently, the analogues LongR3 IGF-I, Des1-3 IGF-I and Arg3 IGF-I were investigated also because of their lower affinity for the IGFBPs, while still retaining unaffected affinity for the IGF-I receptor. Results: All growth factors that were analyzed significantly improved the rate of scratch closure in bronchial and tracheal epithelial cells (p ≤ 0.05). In comparison, scratch closure was markedly slower in nasal epithelial cells and IGF-I was the most effective growth factor at effecting scratch closure in these cells. The IGF-I analogues did not significantly improve scratch closure compared with IGF-I, despite the presence of IGFBP-3 in nasal, bronchial, and tracheal epithelial cells. Conclusion: Addition of IGF-I to wounded nasal epithelial cells increases the rate of scratch closure and therefore may have potential for improving the healing of the nasal mucosa.
dc.description.statementofresponsibilityAdams, Damian H.; McIntosh, David; Wormald, Peter-John; Cowin, Allison J.
dc.description.urihttp://www.oceansidepubl.com/ajr/index.htm
dc.identifier.citationAmerican Journal of Rhinology and Allergy, 2006; 20(6):652-657
dc.identifier.doi10.2500/ajr.2006.20.2916
dc.identifier.issn1945-8924
dc.identifier.issn1050-6586
dc.identifier.orcidWormald, P. [0000-0001-7753-7277]
dc.identifier.orcidCowin, A. [0000-0003-2885-2080]
dc.identifier.urihttp://hdl.handle.net/2440/35785
dc.language.isoen
dc.publisherOcean Side Publications Inc
dc.source.urihttps://doi.org/10.2500/ajr.2006.20.2916
dc.subjectRespiratory Mucosa
dc.subjectCell Line
dc.subjectEpithelial Cells
dc.subjectHumans
dc.subjectWounds and Injuries
dc.subjectInsulin-Like Growth Factor I
dc.subjectInsulin-Like Growth Factor II
dc.subjectWound Healing
dc.subjectCell Movement
dc.titleDifferential effects of insulin-like growth factors on scratch wound repair in respiratory epithelial cells
dc.typeJournal article
pubs.publication-statusPublished

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