The bacterial cytochrome P450 (CYP) CYP125 enzymes can competitively oxidise sitosterol in the presence of cholesterol

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dc.contributor.authorDoherty, D.Z.
dc.contributor.authorGhith, A.
dc.contributor.authorHo, A.
dc.contributor.authorDe Voss, J.J.
dc.contributor.authorBell, S.G.
dc.date.issued2023
dc.description.abstractCholesterol catabolism is an important survival mechanism for the pathogenic Mycobacterium tuberculosis. Various other mycobacteria degrade not only cholesterol but plant sterols such as sitosterol and campesterol. In this work we demonstrate that the cytochrome P450 (CYP) CYP125 enzyme family is capable of sitosterol and campesterol side-chain oxidation and activation in these bacteria. We also show that the CYP142 and CYP124 cholesterol hydroxylating enzyme families are significantly less active for sitosterol hydroxylation compared to CYP125 enzymes.
dc.description.statementofresponsibilityDaniel Z. Doherty, Amna Ghith, Ava Ho, James J. De Voss and Stephen G. Bell
dc.identifier.citationChemical Communications, 2023; 59(61):9392-9395
dc.identifier.doi10.1039/d3cc02312e
dc.identifier.issn1359-7345
dc.identifier.issn1364-548X
dc.identifier.orcidGhith, A. [0000-0003-1753-6939]
dc.identifier.orcidBell, S.G. [0000-0002-7457-9727]
dc.identifier.urihttps://hdl.handle.net/2440/139080
dc.language.isoen
dc.publisherRoyal Society of Chemistry
dc.relation.granthttp://purl.org/au-research/grants/arc/DP210103970
dc.rights© The Royal Society of Chemistry 2023.
dc.source.urihttps://doi.org/10.1039/d3cc02312e
dc.subjectMycobacterium tuberculosis
dc.subjectCholesterol
dc.subjectSitosterols
dc.subjectCytochrome P-450 Enzyme System
dc.subjectOxidation-Reduction
dc.subject.meshMycobacterium tuberculosis
dc.subject.meshCholesterol
dc.subject.meshSitosterols
dc.subject.meshCytochrome P-450 Enzyme System
dc.subject.meshOxidation-Reduction
dc.titleThe bacterial cytochrome P450 (CYP) CYP125 enzymes can competitively oxidise sitosterol in the presence of cholesterol
dc.typeJournal article
pubs.publication-statusPublished

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