Dose-dependent reduction of dietary protein-induced colonocyte DNA damage by resistant starch in rats correlates more highly with caecal butyrate than with other short chain fatty acids

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dc.contributor.authorToden, S.
dc.contributor.authorBird, A.
dc.contributor.authorTopping, D.
dc.contributor.authorConlon, M.
dc.date.issued2007
dc.descriptionCopyright © 2007 Landes Bioscience
dc.description.abstractPrevious studies have shown increased levels of colonocyte DNA damage (as measured by the comet assay) and thinning of the colonic mucus layer in rats fed higher dietary protein as casein or red meat with highly digestible starch. Feeding resistant starch (RS) as high amylose maize starch (HAMS) opposed these changes. However, the dietary level of HAMS was relatively high (48% by weight) so this study was conducted to establish whether HAMS had the same effects at lower dietary levels. Adult male rats were fed a diet containing 25% casein with 0%, 10%, 20%, 30% or 40% HAMS for 4 wk. DNA single strand breaks and 8-hydroxyguanosine levels were measured in isolated colonocytes by the comet assay. As expected, comet tail moment was greatest and the mucus barrier thinnest in rats fed 0% HAMS. DNA damage was reduced and the mucus barrier thickened in a logarithmic dose-dependent manner by HAMS. There was no significant difference in 8-hydroxyguanosine between dietary groups. Caecal and fecal short chain fatty acid (SCFA) pools rose with the increased level of dietary HAMS. DNA damage of colonocytes correlated negatively with caecal SCFA but the strongest correlation was with caecal butyrate, which is consistent with the proposed role of this SCFA in promoting a normal cell phenotype. These data show that HAMS prevents protein-induced colonic DNA damage in a dose-dependent manner. Inclusion of 10% HAMS was found to be sufficient to oppose colonocyte DNA damage, and to increase caecal and fecal SCFA pools.
dc.description.statementofresponsibilityShusuke Toden, Anthony R. Bird, David L. Topping and Michael A. Conlon
dc.identifier.citationCancer Biology & Therapy, 2007; 6(2):253-258
dc.identifier.doi10.4161/cbt.6.2.3627
dc.identifier.issn1538-4047
dc.identifier.issn1555-8576
dc.identifier.urihttp://hdl.handle.net/2440/45688
dc.language.isoen
dc.publisherLandes Bioscience
dc.source.urihttp://www.landesbioscience.com/journals/cbt/article/3627
dc.subjectIntestinal Mucosa
dc.subjectIntestine, Large
dc.subjectAnimals
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.subjectZea mays
dc.subjectIntestinal Diseases
dc.subjectDNA Damage
dc.subjectButyrates
dc.subjectStarch
dc.subjectDietary Carbohydrates
dc.subjectFatty Acids, Volatile
dc.subjectDietary Proteins
dc.subjectPlant Preparations
dc.subjectPhytotherapy
dc.subjectDose-Response Relationship, Drug
dc.subjectMale
dc.subjectEdible Grain
dc.titleDose-dependent reduction of dietary protein-induced colonocyte DNA damage by resistant starch in rats correlates more highly with caecal butyrate than with other short chain fatty acids
dc.typeJournal article
pubs.publication-statusPublished

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