Removal of erythroid cells from umbilical cord blood mononuclear cell preparations using magnetic beads and a monoclonal antibody against glycophorin A

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dc.contributor.authorElliott, S.
dc.contributor.authorMacardle, P.
dc.contributor.authorZola, H.
dc.date.issued1998
dc.description.abstractUmbilical cord blood mononuclear cells isolated by density centrifugation are contaminated by erythrocytes and nucleated erythroid precursors which may exceed 50% of the total cell population, and thus interfere with phenotypic, functional and mRNA analyses. Lysis with hypotonic ammonium chloride can overcome this problem, but interferes with lysosomal function and should be avoided when cell preparations are intended for functional studies. The aim of this study was to develop a technique for removing erythroid cells from cord blood mononuclear cell preparations that would be as effective as ammonium chloride lysis but would not affect cellular function. This paper describes a method using 10F7, a mouse monoclonal antibody against human glycophorin A, and magnetic beads coated with anti-mouse immunoglobulin. The population of cord blood mononuclear cells recovered using this technique was of high purity, good yield and viability, and the cells responded appropriately to stimulation in vitro. To maximise cost-effectiveness, purification with magnetic beads could be performed after two density separations to reduce the quantity of beads required.
dc.identifier.citationJournal of Immunological Methods, 1998; 217(1-2):121-130
dc.identifier.doi10.1016/S0022-1759(98)00111-2
dc.identifier.issn0022-1759
dc.identifier.issn1872-7905
dc.identifier.urihttp://hdl.handle.net/2440/7500
dc.language.isoen
dc.publisherElsevier BV
dc.source.urihttps://doi.org/10.1016/s0022-1759(98)00111-2
dc.subjectErythrocytes
dc.subjectLeukocytes, Mononuclear
dc.subjectFetal Blood
dc.subjectAnimals
dc.subjectHumans
dc.subjectMice
dc.subjectAmmonium Chloride
dc.subjectAntibodies, Monoclonal
dc.subjectHypotonic Solutions
dc.subjectOsmotic Fragility
dc.subjectCentrifugation, Density Gradient
dc.subjectCell Separation
dc.subjectImmunomagnetic Separation
dc.subjectInfant, Newborn
dc.subjectEvaluation Studies as Topic
dc.subjectGlycophorins
dc.titleRemoval of erythroid cells from umbilical cord blood mononuclear cell preparations using magnetic beads and a monoclonal antibody against glycophorin A
dc.typeJournal article
pubs.publication-statusPublished

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