Electrokinetic traps for integrated biological sample analysis
Date
2014
Authors
Shallan, A.I.
Guijt, R.M.
Breadmore, M.C.
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Conference paper
Citation
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, 2014, pp.1467-1469
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18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (26 Oct 2014 - 30 Oct 2014 : San Antonio, USA)
Abstract
A new device integrating diverse electrokinetic steps is presented to extract and enrich analytes directly from blood followed by electrophoretic separation. Two sequentially coupled nanojunctions with different pore size formed a trap with selective ion transport properties. The balance between depletion forces near the nanojunctions and/or apparent electromigration can be tuned for analysis of proteins, DNA, or pharmaceuticals. Here, we demonstrate the analysis of ampicillin from blood within 5 min. The obtained linear range of 2.5-20 μg/mL covers the therapeutically relevant concentration for treating sepsis.
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Copyright 2014 CBMS