Beta-oxidation is essential for mouse oocyte developmental competence and early embryo development

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dc.contributor.authorDunning, K.
dc.contributor.authorCashman, K.
dc.contributor.authorRussell, D.
dc.contributor.authorThompson, J.
dc.contributor.authorNorman, R.
dc.contributor.authorRobker, R.
dc.date.issued2010
dc.description.abstractOocyte and embryo metabolism are closely linked with their subsequent developmental capacity. Lipids are a potent source of cellular energy, yet little is known about lipid metabolism during oocyte maturation and early embryo development. Generation of ATP from lipids occurs within mitochondria via beta-oxidation of fatty acids, with the rate-limiting step catalyzed by carnitine palmitoyl transferase I (CPT1B), a process also requiring carnitine. We sought to investigate the regulation and role of beta-oxidation during oocyte maturation and preimplantation development. Expression of Cpt1b mRNA, assessed by real-time RT-PCR in murine cumulus-oocyte complexes (COCs), increased following hormonal induction of oocyte maturation and ovulation in vivo with human chorionic gonadotropin (5 IU) and in embryos reaching the blastocyst stage. Beta-oxidation, measured by the production of 3H2O from [3H]palmitic acid, was significantly increased over that in immature COCs following induction of maturation in vitro with epidermal growth factor (3 ng/ml) and follicle-stimulating hormone (50 mIU/ml). The importance of lipid metabolism for oocyte developmental competence and early embryo development was demonstrated by assessing the rate of embryo development following inhibition or upregulation of beta-oxidation with etomoxir (an inhibitor of CPT1B) or l-carnitine, respectively. Inhibition of beta-oxidation during oocyte maturation or zygote cleavage impaired subsequent blastocyst development. In contrast, l-carnitine supplementation during oocyte maturation significantly increased beta-oxidation, improved developmental competence, and in the absence of a carbohydrate energy supply, significantly increased 2-cell cleavage. Thus, carnitine is an important cofactor for developing oocytes, and fatty acids are an important energy source for oocyte and embryo development.
dc.description.statementofresponsibilityKylie R. Dunning, Kara Cashman, Darryl L. Russell, Jeremy G. Thompson, Robert J. Norman, and Rebecca L. Robker
dc.identifier.citationBiology of Reproduction, 2010; 83(6):909-918
dc.identifier.doi10.1095/biolreprod.110.084145
dc.identifier.issn0006-3363
dc.identifier.issn1529-7268
dc.identifier.orcidDunning, K. [0000-0002-0462-6479]
dc.identifier.orcidRussell, D. [0000-0002-4930-7658]
dc.identifier.orcidThompson, J. [0000-0003-4941-7731]
dc.identifier.orcidNorman, R. [0000-0002-3118-3896]
dc.identifier.orcidRobker, R. [0000-0002-1538-4604]
dc.identifier.urihttp://hdl.handle.net/2440/62284
dc.language.isoen
dc.publisherSoc Study Reproduction
dc.rights© 2010 by the Society for the Study of Reproduction, Inc.
dc.source.urihttps://doi.org/10.1095/biolreprod.110.084145
dc.subjectbeta-oxidation
dc.subjectCPT1B
dc.subjectembryo development
dc.subjectfatty acid oxidation
dc.subjectoocyte maturation
dc.titleBeta-oxidation is essential for mouse oocyte developmental competence and early embryo development
dc.typeJournal article
pubs.publication-statusPublished

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