Analysis of Drosophila Cyclin EI and II function during development: Identification of an inhibitory zone within the morphogenetic furrow of the eye imaginal disc that blocks the function of Cyclin EI but not Cyclin EII

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dc.contributor.authorDenton, D.
dc.contributor.authorSecombe, J.
dc.contributor.authorCoombe, M.
dc.contributor.authorBrumby, A.
dc.contributor.authorSaint, R.
dc.contributor.authorRichardson, H.
dc.contributor.organisationCentre for the Molecular Genetics of Development
dc.date.issued2002
dc.description.abstractThe Drosophilacyclin E (DmcycE) gene gives rise to two transcripts encoding proteins that differ at their N termini, DmcycEII and DmcycEI. This study presents the first in vivo dissection of Cyclin E function. Ectopic expression studies using N- and C-terminal deletions of DmcycEI revealed that a region of 322 residues surrounding the cyclin box is sufficient to induce entry of G₁-arrested larval eye imaginal disc cells into S phase. Ectopic expression of DmcycEI in the eye disc has been previously shown to drive anterior, but not posterior, G₁-phase cells within the morphogenetic furrow (MF) into S phase. Significantly, ectopic expression of DmcycEII and N-terminal deletions of DmcycEI were able to drive all G₁ cells within the morphogenetic furrow into S phase, while a C-terminal deletion of DmcycEI could not. The p21 homolog Dacapo was shown by yeast two-hybrid, coimmunolocalization, and in vivo functional studies not to be the mediator of the DmcycEI inhibition in posterior part of the MF. Taken together, these results reveal a novel zone within the posterior region of the MF where DmcycEI but not DmcycEII function is inhibited, and suggest that DmcycEII is a more potent inducer of S phase.
dc.description.urihttp://www.sciencedirect.com/science/journal/00121606
dc.identifier.citationDevelopmental Biology, 2002; 241(1):157-171
dc.identifier.doi10.1006/dbio.2001.0496
dc.identifier.issn0012-1606
dc.identifier.issn1095-564X
dc.identifier.urihttp://hdl.handle.net/2440/28134
dc.language.isoen
dc.publisherAcademic Press Inc
dc.relation.granthttp://purl.org/au-research/grants/arc/A09601106
dc.source.urihttps://doi.org/10.1006/dbio.2001.0496
dc.subjectCyclin E
dc.subjectDacapo
dc.subjectG1-S phase
dc.subjectcell cycle
dc.subjectDrosophila
dc.subjectdevelopment
dc.titleAnalysis of Drosophila Cyclin EI and II function during development: Identification of an inhibitory zone within the morphogenetic furrow of the eye imaginal disc that blocks the function of Cyclin EI but not Cyclin EII
dc.typeJournal article
pubs.publication-statusPublished

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