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An enhanced staining method K-B-2R staining for three-dimensional nerve reconstruction

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dc.contributor.authorLuo, P.
dc.contributor.authorDong, J.
dc.contributor.authorQi, J.
dc.contributor.authorZhang, Y.
dc.contributor.authorLiu, X.
dc.contributor.authorZhong, Y.
dc.contributor.authorXian, C.J.
dc.contributor.authorWang, L.
dc.date.issued2019
dc.description.abstractBACKGROUND:Three-dimensional (3D) reconstruction of human peripheral nerves, as a useful tool to understand the nerve internal information and functional basis, has become an important area of research in the peripheral nerve field. METHODS:In this study, we proposed a two-dimensional (2D) Karnovsky-Roots toluidine blue ponceau 2R (K-B-2R) staining method based upon conventional Karnovsky-Roots staining. It significantly improved the ability to display nerve fascicles, motor and sensory nerve fiber textures. In this method, Karnovsky-Roots staining was carried out, followed by toluidine blue counterstain and ponceau 2R counterstain. RESULTS:Comparisons were conducted between the three methods in staining of median nerve sections, which showed similar distribution characters in acetylcholinesterase-positive sites. The additional counterstaining did not change the basis of Karnovsky-Roots staining. However, the resulting images from this new method significantly facilitated the subsequent 3D nerve reconstruction and 3D printing. CONCLUSIONS:These results show that the new staining method significantly enhanced the display qualities of nerve fascicle edges and fiber textures of motor and sensory nerves and facilitated 3D nerve reconstruction.
dc.description.statementofresponsibilityPeng Luo, Jianghui Dong, Jian Qi, Yi Zhang, Xiaolin Liu, Yingchun Zhong, Cory J. Xian and Liping Wang
dc.identifier.citationBMC Neuroscience, 2019; 20(1):32-32
dc.identifier.doi10.1186/s12868-019-0515-7
dc.identifier.issn1471-2202
dc.identifier.issn1471-2202
dc.identifier.orcidXian, C.J. [0000-0002-8467-2845]
dc.identifier.urihttp://hdl.handle.net/2440/120482
dc.language.isoen
dc.publisherSpringer Nature
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1158402
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1127396
dc.rights© The Author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creat iveco mmons .org/licen ses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creat iveco mmons .org/ publi cdoma in/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
dc.source.urihttps://doi.org/10.1186/s12868-019-0515-7
dc.subjectAcetylcholinesterase staining
dc.subjectFascicle texture feature
dc.subjectImage segmentation
dc.subjectK-B-2R
dc.subjectPeripheral nerves
dc.subjectThree-dimensional reconstruction
dc.titleAn enhanced staining method K-B-2R staining for three-dimensional nerve reconstruction
dc.typeJournal article
pubs.publication-statusPublished

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