Differential cohesin loading marks paired and unpaired regions of platypus sex chromosomes at prophase i

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dc.contributor.authorCasey, A.
dc.contributor.authorDaish, T.
dc.contributor.authorBarbero, J.
dc.contributor.authorGrützner, F.
dc.date.issued2017
dc.description.abstractCohesins are vital for chromosome organisation during meiosis and mitosis. In addition to the important function in sister chromatid cohesion, these complexes play key roles in meiotic recombination, DSB repair, homologous chromosome pairing and segregation. Egg-laying mammals (monotremes) feature an unusually complex sex chromosome system, which raises fundamental questions about organisation and segregation during meiosis. We discovered a dynamic and differential accumulation of cohesins on sex chromosomes during platypus prophase I and specific reorganisation of the sex chromosome complex around a large nucleolar body. Detailed analysis revealed a differential loading of SMC3 on the chromatin and chromosomal axis of XY shared regions compared with the chromatin and chromosomal axes of asynapsed X and Y regions during prophase I. At late prophase I, SMC3 accumulation is lost from both the chromatin and chromosome axes of the asynaptic regions of the chain and resolves into subnuclear compartments. This is the first report detailing unpaired DNA specific SMC3 accumulation during meiosis in any species and allows speculation on roles for cohesin in monotreme sex chromosome organisation and segregation.
dc.description.statementofresponsibilityAaron E. Casey, Tasman J. Daish, Jose Luis Barbero, Frank Grützner
dc.identifier.citationScientific Reports, 2017; 7(1):4217-1-4217-14
dc.identifier.doi10.1038/s41598-017-04560-5
dc.identifier.issn2045-2322
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/2440/110158
dc.language.isoen
dc.publisherNature Publishing Group
dc.relation.grantARC
dc.relation.grantBFU2014-59307-R
dc.rights© The Author(s) 2017. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.source.urihttps://doi.org/10.1038/s41598-017-04560-5
dc.subjectSex Chromosomes
dc.subjectChromatin
dc.subjectAnimals
dc.subjectPlatypus
dc.subjectCell Cycle Proteins
dc.subjectChromosomal Proteins, Non-Histone
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectMeiotic Prophase I
dc.subjectChromosome Pairing
dc.subjectFemale
dc.subjectMale
dc.subjectCohesins
dc.titleDifferential cohesin loading marks paired and unpaired regions of platypus sex chromosomes at prophase i
dc.typeJournal article
pubs.publication-statusPublished

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