Multiplex PCR assay for Cylindrospermopsis raciborskii and cylindrospermopsin-producing cyanobacteria
| dc.contributor.author | Ferguson, K.M. | |
| dc.contributor.author | Saint, C. | |
| dc.date.issued | 2003 | |
| dc.description.abstract | Water bodies are routinely monitored for the presence of potentially toxic cyanobacteria; however, the methodology for confirming toxicity is currently complex and expensive. Here we describe the application of gene-based technology to rapidly identify cylindrospermopsin-producing cyanobacteria, specifically, Cylindrospermopsis raciborskii. A multiplex polymerase chain reaction (PCR) test was developed that simultaneously identified polyketide synthase (pks) and peptide synthetase (ps) determinants associated with cylindrospermopsin production and distinguished C. raciborskii from other cylindrospermopsin-producing cyanobacteria of the species Anabaena bergii and Aphanizomenon ovalisporum, by targeting the rpoC1 gene. Twenty-one C. raciborskii, 5 A. bergii, 10 Aph. ovalisporum isolates and 3 environmental samples all yielded PCR results consistent with their toxicological status, as assessed by high-performance liquid chromatography coupled to mass spectrometry or matrix-assisted laser desorption ionization-time-of-flight mass spectrometry, and C. raciborskii was always correctly identified. The PCR test is a rapid, reliable, and economical way of assessing the toxic potential of cyanobacterial blooms formed by these organisms. | |
| dc.identifier.citation | Environmental Toxicology, 2003; 18(2):120-125 | |
| dc.identifier.doi | 10.1002/tox.10108 | |
| dc.identifier.issn | 1520-4081 | |
| dc.identifier.issn | 1522-7278 | |
| dc.identifier.uri | https://hdl.handle.net/1959.8/87251 | |
| dc.language.iso | en | |
| dc.publisher | John Wiley and Sons | |
| dc.rights | Copyright status unknown | |
| dc.source.uri | https://doi.org/10.1002/tox.10108 | |
| dc.subject | toxicology | |
| dc.subject | cylindrospermopsis raciborskii | |
| dc.subject | cylindrospermopsin-producing | |
| dc.subject | cyanobacteria | |
| dc.title | Multiplex PCR assay for Cylindrospermopsis raciborskii and cylindrospermopsin-producing cyanobacteria | |
| dc.type | Journal article | |
| pubs.publication-status | Published | |
| ror.mmsid | 9915913486301831 |