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Vacuolar glyphosate-sequestration correlates with glyphosate resistance in ryegrass (Lolium spp.) from Australia, South America, and Europe: A (31)P NMR investigation

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dc.contributor.authorGe, X.
dc.contributor.authord'Avignon, D.
dc.contributor.authorAckerman, J.
dc.contributor.authorCollavo, A.
dc.contributor.authorSattin, M.
dc.contributor.authorOstrander, E.
dc.contributor.authorHall, E.
dc.contributor.authorSammons, R.
dc.contributor.authorPreston, C.
dc.date.issued2012
dc.description.abstractLolium spp., ryegrass, variants from Australia, Brazil, Chile, and Italy showing differing levels of glyphosate resistance were examined by (31)P NMR. Extents of glyphosate (i) resistance (LD(50)), (ii) inhibition of 5-enopyruvyl-shikimate-3-phosphate synthase (EPSPS) activity (IC(50)), and (iii) translocation were quantified for glyphosate-resistant (GR) and glyphosate-sensitive (GS) Lolium multiflorum Lam. variants from Chile and Brazil. For comparison, LD(50) and IC(50) data for Lolium rigidum Gaudin variants from Italy were also analyzed. All variants showed similar cellular uptake of glyphosate by (31)P NMR. All GR variants showed glyphosate sequestration within the cell vacuole, whereas there was minimal or no vacuole sequestration in the GS variants. The extent of vacuole sequestration correlated qualitatively with the level of resistance. Previous (31)P NMR studies of horseweed ( Conyza canadensis (L.) Cronquist) revealed that glyphosate sequestration imparted glyphosate resistance. Data presented herein suggest that glyphosate vacuolar sequestration is strongly contributing, if not the major contributing, resistance mechanism in ryegrass as well.
dc.description.statementofresponsibilityXia Ge, D. André d’Avignon, Joseph J. H. Ackerman, Alberto Collavo, Maurizio Sattin, Elizabeth L. Ostrander, Erin L. Hall, R. Douglas Sammons and Christopher Preston
dc.identifier.citationJournal of Agricultural and Food Chemistry, 2012; 60(5):1243-1250
dc.identifier.doi10.1021/jf203472s
dc.identifier.issn0021-8561
dc.identifier.issn1520-5118
dc.identifier.orcidPreston, C. [0000-0002-7588-124X]
dc.identifier.urihttp://hdl.handle.net/2440/71623
dc.language.isoen
dc.publisherAmer Chemical Soc
dc.rightsCopyright © 2012 American Chemical Society
dc.source.urihttps://doi.org/10.1021/jf203472s
dc.subjectGlyphosate
dc.subjectglyphosate resistance
dc.subjectin vivo 31P NMR
dc.subjectLolium spp.
dc.subjectglyphosate tonoplast transporter
dc.titleVacuolar glyphosate-sequestration correlates with glyphosate resistance in ryegrass (Lolium spp.) from Australia, South America, and Europe: A (31)P NMR investigation
dc.typeJournal article
pubs.publication-statusPublished

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