A simplified point-of-care testing approach for preeclampsia blood biomarkers based on nanoscale field effect transistors

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dc.contributor.authorPham, T.T.T.
dc.contributor.authorTran, D.P.
dc.contributor.authorNguyen, M.C.
dc.contributor.authorAmen, M.T.
dc.contributor.authorWinter, M.
dc.contributor.authorWhitehead, C.
dc.contributor.authorToh, J.
dc.contributor.authorThierry, B.
dc.date.issued2021
dc.description.abstractRapid diagnosis of preeclampsia is necessary to ensure timely administration of appropriate care and prevent the potentially catastrophic complications of the condition affecting both mothers and babies. While the diagnostic superiority of angiogenic blood biomarkers such as placental growth factor has recently been demonstrated, there is an urgent need to develop point-of-care (PoC) technologies that allow rapid, quantitative, and accurate testing for these markers within local communities. Towards addressing this need, here we report on a fully integrated biodiagnostic platform based on nanoscale indium oxide field effect transistor (FET) sensors. The high-performance FET sensors are integrated with blood sample processing cartridges that minimize the need for operator intervention during the assay and eliminate the need for analytical equipment. Within 40 minutes and from 30 μL of blood, the FET platform could reliably measure PlGF with a limit of detection of 0.06 pg mL<sup>-1</sup> and a five order of magnitudes dynamic range. Pilot clinical validation in four preeclamptic pregnancies confirmed that the accuracy and reliability of the FET platform, paving the way for further development to a much-needed point-of-care preeclampsia testing.
dc.description.statementofresponsibilityThuy Thi Thanh Pham, Duy Phu Tran, Minh Cong Nguyen, Mohamed Taha Amen, Marnie Winter, Clare Whitehead ... et al.
dc.identifier.citationNanoscale, 2021; 13(28):12279-12287
dc.identifier.doi10.1039/d1nr02461b
dc.identifier.issn2040-3364
dc.identifier.issn2040-3372
dc.identifier.urihttps://hdl.handle.net/2440/132858
dc.language.isoen
dc.publisherRoyal Society of Chemistry
dc.relation.granthttp://purl.org/au-research/grants/arc/DP170100170
dc.source.urihttps://doi.org/10.1039/d1nr02461b
dc.subjectHumans
dc.subjectPre-Eclampsia
dc.subjectReproducibility of Results
dc.subjectPregnancy
dc.subjectFemale
dc.subjectBiomarkers
dc.subjectPoint-of-Care Testing
dc.subjectPlacenta Growth Factor
dc.subject.meshHumans
dc.subject.meshPre-Eclampsia
dc.subject.meshReproducibility of Results
dc.subject.meshPregnancy
dc.subject.meshFemale
dc.subject.meshBiomarkers
dc.subject.meshPoint-of-Care Testing
dc.subject.meshPlacenta Growth Factor
dc.titleA simplified point-of-care testing approach for preeclampsia blood biomarkers based on nanoscale field effect transistors
dc.typeJournal article
pubs.publication-statusPublished

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