Vasculogenic properties of adventitial Sca-1⁺CD45⁺ progenitor cells in mice: a potential source of vasa vasorum in atherosclerosis

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dc.contributor.authorToledo-Flores, D.
dc.contributor.authorWilliamson, A.
dc.contributor.authorSchwarz, N.
dc.contributor.authorFernando, S.
dc.contributor.authorDimasi, C.
dc.contributor.authorWitt, T.A.
dc.contributor.authorNguyen, T.M.
dc.contributor.authorPuranik, A.S.
dc.contributor.authorChue, C.D.
dc.contributor.authorDelacroix, S.
dc.contributor.authorSpoon, D.B.
dc.contributor.authorBonder, C.S.
dc.contributor.authorBursill, C.A.
dc.contributor.authorDi Bartolo, B.A.
dc.contributor.authorNicholls, S.J.
dc.contributor.authorSimari, R.D.
dc.contributor.authorPsaltis, P.J.
dc.date.issued2019
dc.description.abstractThe cellular origins of vasa vasorum are ill-defined and may involve circulating or local progenitor cells. We previously discovered that murine aortic adventitia contains Sca-1⁺CD45⁺ progenitors that produce macrophages. Here we investigated whether they are also vasculogenic. In aortas of C57BL/6 mice, Sca-1⁺CD45⁺ cells were localised to adventitia and lacked surface expression of endothelial markers (<1% for CD31, CD144, TIE-2). In contrast, they did show expression of CD31, CD144, TIE-2 and VEGFR2 in atherosclerotic ApoE(-/-) aortas. Although Sca-1⁺CD45⁺ cells from C57BL/6 aorta did not express CD31, they formed CD31⁺ colonies in endothelial differentiation media and produced interconnecting vascular-like cords in Matrigel that contained both endothelial cells and a small population of macrophages, which were located at branch points. Transfer of aortic Sca-1⁺CD45⁺ cells generated endothelial cells and neovessels de novo in a hindlimb model of ischaemia and resulted in a 50% increase in perfusion compared to cell-free control. Similarly, their injection into the carotid adventitia of ApoE(-/-) mice produced donor-derived adventitial and peri-adventitial microvessels after atherogenic diet, suggestive of newly formed vasa vasorum. These findings show that beyond its content of macrophage progenitors, adventitial Sca-1⁺CD45⁺ cells are also vasculogenic and may be a source of vasa vasorum during atherogenesis.
dc.description.statementofresponsibilityDeborah Toledo-Flores, Anna Williamson, Nisha Schwarz, Sanuja Fernando, Catherine Dimasi, Tyra A. Witt, Thao M. Nguyen, Amrutesh S . Puranik, Colin D. Chue, Sinny Delacroix, Daniel B. Spoon, Claudine S. Bonder, Christina A. Bursill, Belinda A. Di Bartolo, Stephen J. Nicholls, Robert D. Simari, Peter J. Psaltis
dc.identifier.citationScientific Reports, 2019; 9(1):7286-1-7286-15
dc.identifier.doi10.1038/s41598-019-43765-8
dc.identifier.issn2045-2322
dc.identifier.issn2045-2322
dc.identifier.orcidWilliamson, A. [0000-0003-3446-9248]
dc.identifier.orcidFernando, S. [0000-0002-4766-2250]
dc.identifier.orcidNguyen, T.M. [0000-0001-9066-6962]
dc.identifier.orcidBonder, C.S. [0000-0001-9875-967X]
dc.identifier.orcidBursill, C.A. [0000-0002-0682-8760] [0000-0003-1087-7781]
dc.identifier.orcidDi Bartolo, B.A. [0000-0001-8420-0461]
dc.identifier.orcidNicholls, S.J. [0000-0002-9668-4368]
dc.identifier.orcidPsaltis, P.J. [0000-0003-0222-5468]
dc.identifier.urihttps://hdl.handle.net/2440/132080
dc.language.isoen
dc.publisherSpringer Nature
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1086796
dc.rights© The Author(s) 2019. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.source.urihttps://doi.org/10.1038/s41598-019-43765-8
dc.subjectVascular system
dc.subject.meshAorta
dc.subject.meshVasa Vasorum
dc.subject.meshMacrophages
dc.subject.meshEndothelial Cells
dc.subject.meshStem Cells
dc.subject.meshAnimals
dc.subject.meshHumans
dc.subject.meshMice
dc.subject.meshDisease Models, Animal
dc.subject.meshNeovascularization, Pathologic
dc.subject.meshMembrane Proteins
dc.subject.meshAntigens, Ly
dc.subject.meshDiet, Atherogenic
dc.subject.meshCell Differentiation
dc.subject.meshFemale
dc.subject.meshMale
dc.subject.meshAtherosclerosis
dc.subject.meshAdventitia
dc.subject.meshMice, Knockout, ApoE
dc.subject.meshLeukocyte Common Antigens
dc.titleVasculogenic properties of adventitial Sca-1⁺CD45⁺ progenitor cells in mice: a potential source of vasa vasorum in atherosclerosis
dc.title.alternativeVasculogenic properties of adventitial Sca-1(+)CD45(+) progenitor cells in mice: a potential source of vasa vasorum in atherosclerosis
dc.typeJournal article
pubs.publication-statusPublished

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