Applying nanoparticle tracking analysis to characterize the polydispersity of aggregates resulting from tannin-polysaccharide interactions in wine-like media

Simple item page
dc.contributor.authorLi, S.
dc.contributor.authorWilkinson, K.L.
dc.contributor.authorMierczynska-Vasilev, A.
dc.contributor.authorBindon, K.A.
dc.date.issued2019
dc.descriptionPublished: 3 June 2019
dc.description.abstractInteractions between grape seed tannin and either a mannoprotein or an arabinogalactan in model wine solutions of different ethanol concentrations were characterized with nanoparticle tracking analysis (NTA), UV-visible spectroscopy and dynamic light scattering (DLS). NTA results reflected a shift in particle size distribution due to aggregation. Furthermore, the light scattering intensity of each tracked particle measured by NTA demonstrated the presence of aggregates, even when a shift in particle size was not apparent. Mannoprotein and arabinogalactan behaved differently when combined with seed tannin. Mannoprotein formed large, highly light-scattering aggregates, while arabinogalactan exhibited only weak interactions with seed tannin. A 3% difference in alcohol concentration of the model solution (12 vs. 15% v/v) was sufficient to affect the interactions between mannoprotein and tannin when the tannin concentration was high. In summary, this study showed that NTA is a promising tool for measuring polydisperse samples of grape and wine macromolecules, and their aggregates under wine-like conditions. The implications for wine colloidal properties are discussed based on these results.
dc.description.statementofresponsibilitySijing Li, Kerry L. Wilkinson, Agnieszka Mierczynska-Vasilev and Keren A. Bindon
dc.identifier.citationMolecules, 2019; 24(11):2100-1-2100-18
dc.identifier.doi10.3390/molecules24112100
dc.identifier.issn1420-3049
dc.identifier.issn1420-3049
dc.identifier.orcidLi, S. [0000-0002-0246-8948]
dc.identifier.orcidWilkinson, K.L. [0000-0001-6724-9837]
dc.identifier.urihttp://hdl.handle.net/2440/131603
dc.language.isoen
dc.publisherMDPI AG
dc.relation.granthttp://purl.org/au-research/grants/arc/IC130100005
dc.rights© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
dc.source.urihttps://doi.org/10.3390/molecules24112100
dc.subjectmannoprotein
dc.subjectarabinogalactan
dc.subjectseed tannin
dc.subjectaggregation
dc.subjectnanoparticle tracking analysis
dc.titleApplying nanoparticle tracking analysis to characterize the polydispersity of aggregates resulting from tannin-polysaccharide interactions in wine-like media
dc.typeJournal article
pubs.publication-statusPublished

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
hdl_131603.pdf
Size:
1.31 MB
Format:
Adobe Portable Document Format
Description:
Published version
Download

Collections

Agriculture, Food and Wine publications
ARC Training Centre for Innovative Wine Production publications