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Antibacterial activity of reduced graphene oxide prepared by microbe

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dc.contributor.authorZhou, Y.
dc.contributor.authorXue, C.
dc.contributor.authorGan, L.
dc.contributor.authorOwens, G.
dc.contributor.authorChen, Z.
dc.date.issued2023
dc.description.abstractIn previous work, we successfully bioreduced graphene oxide (B-rGO) utilizing organisms, but the antibacterial properties of B-rGO are still unknown. In this study, Escherichia coli (E. coli) was used as a test target and the antibacterial activity of B-rGO was compared to that of commercial reduced graphene oxide (C-rGO), in order to understand its toxicity. Data from levels of extracellular lactate dehydrogenase (LDH), and intracellular reactive oxygen species (ROS) content demonstrate that cell membrane stress and oxidative stress play a role in this antibacterial process. However, when the concentration of co-existing B-rGO was 50.0 mg/L, E. coli damaged easily, the LDH release concentration was 98.75 U/L, the ROS fluorescence signal was 57.75/106 cells, and the phenomenon known as ‘wrapping’ occurred. Indicated here is that B-rGO does indeed demonstrate very good antibacterial activity as evidenced when using Scanning Electron Microscopy (SEM). Meanwhile, numerous oxygen-containing functional groups existed on the surface of B-rGO as confirmed by Fourier transform infrared spectroscopy (FTIR). The power-time curve of microcalorimetry and flow cytometry analysis confirmed that the antibacterial activity of B-rGO was inadequate when compared to that of C-rGO. Despite E. coli mortality being dosage-dependent for both B-rGO and C-rGO, this implies that B-rGO does in fact achieve good biocompatibility. The outcomes reveal that B-rGO has great potential for environmental remediation.
dc.identifier.citationMaterials Today Sustainability, 2023; 22(100341)
dc.identifier.doi10.1016/j.mtsust.2023.100341
dc.identifier.issn2589-2347
dc.identifier.issn2589-2347
dc.identifier.orcidOwens, G. [0000-0002-4606-3678]
dc.identifier.urihttps://hdl.handle.net/11541.2/33072
dc.language.isoen
dc.publisherELSEVIER
dc.relation.fundingNational Natural Science Foundation of China 51978168
dc.relation.fundingNatural Science Foundation of Fujian Province 2020J01199
dc.rightsCopyright 2023 Elsevier Ltd. All rights reserved
dc.source.urihttps://doi.org/10.1016/j.mtsust.2023.100341
dc.subjectantibacterial activity
dc.subjectEscherichia coli
dc.subjectreduced graphene oxide
dc.titleAntibacterial activity of reduced graphene oxide prepared by microbe
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9916721928901831

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