Lipoxygenase in wheat: Genetic control and impact on stability of lutein and lutein esters

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dc.contributor.authorMares, D.J.
dc.contributor.authorCheong, J.
dc.contributor.authorGoonetilleke, W.
dc.contributor.authorMather, D.E.
dc.date.issued2021
dc.descriptionPublished: 20 May 2021
dc.description.abstractPreservation of lutein concentrations in wheat-based end-products during processing is important both for product quality and nutritional value. A key constituent involved in lutein degradation is endogenous lipoxygenase. Lutein and lutein ester concentrations were compared at intervals during storage of noodle sheets prepared from flour of wheat varieties representing a range in lipoxygenase activity, as well as in different mill streams and in different grain tissues. Higher lipoxygenase concentration was associated with an increased loss of free lutein and lutein mono-esters whereas lutein diesters appeared to be more resistant to degradation. Lutein degradation was reduced in the presence of a lipoxygenase inhibitor, when noodle sheets were heated to destroy enzyme activity or when pH was increased. In addition, three populations were used to investigate the genetic control of lipoxygenase. A previously reported mutation of Lpx-B1.1 was associated with a reduction in activity from high to intermediate whilst a new locus on chromosome 4D was associated with variation between intermediate and near-zero. The gene underlying the 4D locus is a putative lipoxygenase. Stability of lutein could be improved by deployment of the mutations at the 4B and 4D loci and/or by post-harvest storage of grain under conditions that promote esterification.
dc.description.statementofresponsibilityDaryl J. Mares, Judy Cheong, Shashi N. Goonetilleke and Diane E. Mather
dc.identifier.citationFoods, 2021; 10(5):1149-1-1149-12
dc.identifier.doi10.3390/foods10051149
dc.identifier.issn2304-8158
dc.identifier.issn2304-8158
dc.identifier.orcidMares, D.J. [0000-0002-8165-8140]
dc.identifier.orcidGoonetilleke, W. [0000-0001-7539-011X]
dc.identifier.orcidMather, D.E. [0000-0001-7506-2589]
dc.identifier.urihttps://hdl.handle.net/2440/133322
dc.language.isoen
dc.publisherMDPI AG
dc.rightsCopyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
dc.source.urihttps://doi.org/10.3390/foods10051149
dc.subjectlipoxygenase genes; Asian noodle colour; genetic loci; molecular markers
dc.titleLipoxygenase in wheat: Genetic control and impact on stability of lutein and lutein esters
dc.typeJournal article
pubs.publication-statusPublished

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