Production of a monoclonal antibody to human interferon-α (IFN-α) and its use to identify IFN-α-producing cells in virus infection in vivo

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dc.contributor.authorJilbert, A.R.
dc.contributor.authorHertzog, P.J.
dc.contributor.authorBurrell, C.J.
dc.contributor.authorGowans, E.J.
dc.contributor.authorLinnane, A.W.
dc.contributor.authorMarmion, B.P.
dc.date.issued1986
dc.description.abstractA monoclonal antibody to human interferon-alpha (IFN-alpha) was produced using affinity-purified IFN-alpha, that reacted with recombinant human IFN-alpha 2, but not with IFN-alpha 1, IFN-alpha M1 or IFN-beta. Indirect immunofluorescence using this monoclonal (designated 6C3) and anti-IFN-alpha polyclonal antibodies identified cells expressing IFN-alpha. After Sendai virus induction of normal human buffy-coat cells the proportion of monocytes and lymphocytes expressing IFN-alpha rose progressively from 0% to 50% and 34% respectively, preceding peak IFN-alpha titres in the culture supernatants. Around 80-90% of polymorphs were IFN-alpha-positive using both antisera, with or without IFN induction, although very little IFN bioactivity was released to the supernatant of polymorph cultures after IFN induction. Sections of hepatitis B virus infected human liver tissue showed foci of IFN-alpha-positive infiltrating mononuclear cells and (to a lesser extent) fibroblasts in patients who had active cirrhosis and evidence of virus replication. These findings suggest that polymorphs constitutively express IFN-alpha 2 related antigenic activity, whose biological activity is at present unknown; and demonstrates the identification of IFN-alpha-expressing cells in sections of tissue undergoing natural virus infection.
dc.description.statementofresponsibilityAllison R. Jilbert, Paul J. Hertzog, Christopher J. Burrell, Eric J. Gowans, Anthony W. Linnane, Barrie P. Marmion
dc.identifier.citationMicrobial Pathogenesis, 1986; 1(2):159-168
dc.identifier.doi10.1016/0882-4010(86)90018-5
dc.identifier.issn0882-4010
dc.identifier.issn1096-1208
dc.identifier.orcidJilbert, A.R. [0000-0003-3855-1679]
dc.identifier.orcidBurrell, C.J. [0000-0002-4020-349X]
dc.identifier.orcidGowans, E.J. [0000-0002-4274-8311]
dc.identifier.urihttp://hdl.handle.net/2440/86668
dc.language.isoen
dc.publisherElsevier
dc.rights©1986 Academic Press Inc. (London) Ltd.
dc.source.urihttps://doi.org/10.1016/0882-4010(86)90018-5
dc.subjecthuman interferon-α; monoclonal antibodies; immunofluorescence; hepatitis B virus infection
dc.titleProduction of a monoclonal antibody to human interferon-α (IFN-α) and its use to identify IFN-α-producing cells in virus infection in vivo
dc.title.alternativeProduction of a monoclonal antibody to human interferon-alpha (IFN-alpha) and its use to identify IFN-alpha-producing cells in virus infection in vivo
dc.typeJournal article
pubs.publication-statusPublished

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