NaCl-induced changes in cytosolic free Ca2+ in Arabidopsis thaliana are heterogeneous and modified by external ionic composition

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dc.contributor.authorTracy, F.
dc.contributor.authorGilliham, M.
dc.contributor.authorDodd, A.
dc.contributor.authorWebb, A.
dc.contributor.authorTester, M.
dc.date.issued2008
dc.descriptionThe definitive version may be found at www.wiley.com
dc.description.abstractIncreases in cytosolic free Ca(2+) ([Ca(2+)](cyt)) are common to many stress-activated signalling pathways, including the response to saline environments. We have investigated the nature of NaCl-induced [Ca(2+)](cyt) signals in whole Arabidopsis thaliana seedlings using aequorin. We found that NaCl-induced increases in [Ca(2+)](cyt) are heterogeneous and mainly restricted to the root. Both the concentration of NaCl and the composition of the solution bathing the root have profound effects on the magnitude and dynamics of NaCl-induced increases in [Ca(2+)](cyt). Alteration of external K(+) concentration caused changes in the temporal and spatial pattern of [Ca(2+)](cyt) increase, providing evidence for Na(+)-induced Ca(2+) influx across the plasma membrane. The effects of various pharmacological agents on NaCl-induced increases in [Ca(2+)](cyt) indicate that NaCl may induce influx of Ca(2+) through both plasma membrane and intracellular Ca(2+)-permeable channels. Analysis of spatiotemporal [Ca(2+)](cyt) dynamics using photon-counting imaging revealed additional levels of complexity in the [Ca(2+)](cyt) signal that may reflect the oscillatory nature of NaCl-induced changes in single cells.
dc.description.statementofresponsibilityFrances E. Tracy, Matthew Gilliham, Antony N. Dodd, Alex A. R. Webb & Mark Tester
dc.identifier.citationPlant, Cell and Environment, 2008; 31(8):1063-1073
dc.identifier.doi10.1111/j.1365-3040.2008.01817.x
dc.identifier.issn0140-7791
dc.identifier.issn1365-3040
dc.identifier.orcidGilliham, M. [0000-0003-0666-3078]
dc.identifier.urihttp://hdl.handle.net/2440/53245
dc.language.isoen
dc.publisherBlackwell Publishing Ltd
dc.source.urihttps://doi.org/10.1111/j.1365-3040.2008.01817.x
dc.subjectCytosol
dc.subjectArabidopsis
dc.subjectSodium Chloride
dc.subjectCalcium
dc.subjectSorbitol
dc.subjectNeomycin
dc.subjectSolutions
dc.subjectCalcium Signaling
dc.subjectOsmosis
dc.subjectMembrane Potentials
dc.subjectOsmolar Concentration
dc.subjectPhotons
dc.subjectSeedlings
dc.titleNaCl-induced changes in cytosolic free Ca2+ in Arabidopsis thaliana are heterogeneous and modified by external ionic composition
dc.typeJournal article
pubs.publication-statusPublished

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