CXCR4/CXCL12 participate in extravasation of metastasizing breast cancer cells within the liver in a rat model

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dc.contributor.authorWendel, C.
dc.contributor.authorHemping-Bovenkerk, A.
dc.contributor.authorKrasnyanska, J.
dc.contributor.authorMees, S.
dc.contributor.authorKochetkova, M.
dc.contributor.authorStoeppeler, S.
dc.contributor.authorHaier, J.
dc.contributor.editorAziz, S.A.
dc.date.issued2012
dc.description.abstractINTRODUCTION: Organ-specific composition of extracellular matrix proteins (ECM) is a determinant of metastatic host organ involvement. The chemokine CXCL12 and its receptor CXCR4 play important roles in the colonization of human breast cancer cells to their metastatic target organs. In this study, we investigated the effects of chemokine stimulation on adhesion and migration of different human breast cancer cell lines in vivo and in vitro with particular focus on the liver as a major metastatic site in breast cancer. METHODS: Time lapse microscopy, in vitro adhesion and migration assays were performed under CXCL12 stimulation. Activation of small GTPases showed chemokine receptor signalling dependence from ECM components. The initial events of hepatic colonisation of MDA-MB-231 and MDA-MB-468 cells were investigated by intravital microscopy of the liver in a rat model and under shRNA inhibition of CXCR4. RESULTS: In vitro, stimulation with CXCL12 induced increased chemotactic cell motility (p,0.05). This effect was dependent on adhesive substrates (type I collagen, fibronectin and laminin) and induced different responses in small GTPases, such as RhoA and Rac-1 activation, and changes in cell morphology. In addition, binding to various ECM components caused redistribution of chemokine receptors at tumour cell surfaces. In vivo, blocking CXCR4 decreased extravasation of highly metastatic MDA-MB-231 cells (p < 0.05), but initial cell adhesion within the liver sinusoids was not affected. In contrast, the less metastatic MDA-MB-468 cells showed reduced cell adhesion but similar migration within the hepatic microcirculation. CONCLUSION: Chemokine-induced extravasation of breast cancer cells along specific ECM components appears to be an important regulator but not a rate-limiting factor of their metastatic organ colonization.
dc.description.statementofresponsibilityClaudia Wendel, André Hemping-Bovenkerk, Julia Krasnyanska, Sören Torge Mees, Marina Kochetkova, Sandra Stoeppeler and Jörg Haier
dc.identifier.citationPLoS One, 2012; 7(1):1-13
dc.identifier.doi10.1371/journal.pone.0030046
dc.identifier.issn1932-6203
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/2440/73134
dc.language.isoen
dc.publisherPublic Library of Science
dc.rightsCopyright: © 2012 Wendel et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.source.urihttps://doi.org/10.1371/journal.pone.0030046
dc.subjectCell Line, Tumor
dc.subjectCell Membrane
dc.subjectExtracellular Matrix
dc.subjectAnimals
dc.subjectHumans
dc.subjectRats
dc.subjectLiver Neoplasms
dc.subjectMammary Neoplasms, Animal
dc.subjectDisease Models, Animal
dc.subjectrho GTP-Binding Proteins
dc.subjectReceptors, CXCR4
dc.subjectIntegrins
dc.subjectProtein Subunits
dc.subjectFlow Cytometry
dc.subjectCluster Analysis
dc.subjectCell Adhesion
dc.subjectSignal Transduction
dc.subjectCell Movement
dc.subjectEnzyme Activation
dc.subjectKinetics
dc.subjectFemale
dc.subjectChemokine CXCL12
dc.titleCXCR4/CXCL12 participate in extravasation of metastasizing breast cancer cells within the liver in a rat model
dc.typeJournal article
pubs.publication-statusPublished

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