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Dietary supplementation with zinc and a growth factor extract derived from bovine cheese whey improves methotrexate-damaged rat intestine

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dc.contributor.authorTran, C.
dc.contributor.authorHowarth, G.
dc.contributor.authorCoyle, P.
dc.contributor.authorPhilcox, J.
dc.contributor.authorRofe, A.
dc.contributor.authorButler, R.
dc.date.issued2003
dc.description.abstractBackground: Oral administration of zinc or bovine whey-derived growth factor extract (WGFE) is known to reduce intestinal permeability and ameliorate methotrexate (MTX)-induced mucositis, respectively. Objective: We examined the effects of zinc, WGFE, and zinc plus WGFE on gut damage in MTX-treated rats. Design: Rats (n = 16/group) were fed zinc (1000 mg/kg diet), WGFE (32 mg/kg diet), zinc plus WGFE, or control (10 mg Zn/kg diet) diets for 7 d and then injected subcutaneously with MTX (2.5 mg/kg) for 3 d to induce gut damage. Gut histology and intestinal permeability were assessed. Results: The Zn+WGFE diet was associated with both reduced gut damage on day 5 and enhanced recovery on day 7. The WGFE diet ameliorated gut damage, whereas the Zn and Zn+WGFE diets enhanced repair. Gut metallothionein and tissue zinc concentrations were significantly (P < 0.01) higher with Zn and Zn+WGFE on days 5 and 7 than without zinc supplementation. The Zn and Zn+WGFE diets significantly (P < 0.05) decreased gut permeability on days 3–4 compared with the control diet. Intestinal permeability was significantly (P < 0.05) increased on days 5–6. On days 6–7, only the WGFE diet improved gut permeability (by 80%) compared with the control diet. Conclusions: Dietary administration of WGFE and a pharmacologic dose of zinc reduced intestinal damage and enhanced recovery, respectively. WGFE also improved gut permeability after MTX-induced bowel damage. In combination, zinc and WGFE hastened repair of gut damage, which may have clinical application in chemotherapy-induced mucositis.
dc.description.statementofresponsibilityCuong D Tran, Gordon S Howarth, Peter Coyle, Jeffrey C Philcox, Allan M Rofe, and Ross N Butler
dc.identifier.citationAmerican Journal of Clinical Nutrition, 2003; 77(5):1296-1303
dc.identifier.doi10.1093/ajcn/77.5.1296
dc.identifier.issn0002-9165
dc.identifier.issn1938-3207
dc.identifier.orcidTran, C. [0000-0001-9220-2715]
dc.identifier.orcidHowarth, G. [0000-0001-6979-6084]
dc.identifier.urihttp://hdl.handle.net/2440/2916
dc.language.isoen
dc.publisherAmer Soc Clinical Nutrition
dc.source.urihttp://www.ajcn.org/cgi/content/abstract/77/5/1296
dc.subjectZinc
dc.subjectwhey growth factor extract
dc.subjectintestinal permeability
dc.subjectsmall-intestine damage
dc.titleDietary supplementation with zinc and a growth factor extract derived from bovine cheese whey improves methotrexate-damaged rat intestine
dc.typeJournal article
pubs.publication-statusPublished

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