Alcohol metabolism increases the replication of hepatitis C virus and attenuates the antiviral action of interferon

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dc.contributor.authorMcCartney, E.
dc.contributor.authorSemendric, L.
dc.contributor.authorHelbig, K.
dc.contributor.authorHinze, S.
dc.contributor.authorJones, B.
dc.contributor.authorWeinman, S.
dc.contributor.authorBeard, M.
dc.date.issued2008
dc.description.abstractThe interactions between hepatitis C virus (HCV) and alcohol metabolism are not well understood. To determine the effect that alcohol metabolism has on HCV replication and the antiviral action of interferon (IFN), Huh-7 cells that harbor HCV replication and metabolize ethanol via the introduced expression of cytochrome P450 2E1 (Cyp2e1) were treated with ethanol and IFN-alpha. Treatment of these cells with ethanol (0-100 mmol/L) significantly increased HCV replication. This effect was dependent on Cyp2e1 expression and alcohol-metabolized oxidative stress (OS), because the antioxidant N-acetylcysteine blocked this effect. Furthermore, the anti-HCV action of IFN-alpha was attenuated in the presence of ethanol metabolism, most likely via attenuation of Stat1 tyrosine-701 phosphorylation. These in vitro results mimic what is often noted clinically, and further dissection of this model system will aid in our understanding of interactions between HCV and alcohol metabolism.
dc.description.statementofresponsibilityErin M. McCartney, Ljiljana Semendric, Karla J. Helbig, Susan Hinze, Brett Jones, Steven A. Weinman and Michael R. Beard
dc.identifier.citationJournal of Infectious Diseases, 2008; 198(12):1766-1775
dc.identifier.doi10.1086/593216
dc.identifier.issn0022-1899
dc.identifier.issn1537-6613
dc.identifier.orcidBeard, M. [0000-0002-4106-1016]
dc.identifier.urihttp://hdl.handle.net/2440/51564
dc.language.isoen
dc.publisherUniv Chicago Press
dc.source.urihttps://doi.org/10.1086/593216
dc.subjectCell Line
dc.subjectHumans
dc.subjectHepacivirus
dc.subjectEthanol
dc.subjectCytochrome P-450 CYP2E1
dc.subjectVirus Replication
dc.subjectGene Expression Regulation, Enzymologic
dc.subjectOxidative Stress
dc.titleAlcohol metabolism increases the replication of hepatitis C virus and attenuates the antiviral action of interferon
dc.typeJournal article
pubs.publication-statusPublished

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