Experimental infections, using a fluorescent marker, of two elasmobranch species by unciliated larvae of Branchotenthes octohamatus (Monogenea: Hexabothriidae): invasion route, host specificity and post-larval development

Simple item page
dc.contributor.authorGlennon, V.
dc.contributor.authorChisholm, L.
dc.contributor.authorWhittington, I.
dc.date.issued2007
dc.description.abstractThe infection biology of Branchotenthes octohamatus (Monogenea: Hexabothriidae) from the gills of the southern fiddler ray, Trygonorrhina fasciata (Rhinobatidae), was studied using the fluorescent dye, 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFSE). This is the first use of this technique on a monogenean species with unciliated larvae and the first for any monogenean larva infecting an elasmobranch host. CFSE-labelled post-larvae were recovered from gills of T. fasciata within 30 min of exposure to the host, providing strong evidence that larvae invade host gills directly and do not migrate after initial attachment elsewhere. The rapidity with which larvae settled suggests that the mode of infection may deliver larvae directly to the gills via the host's inhalant respiratory current. The specificity of B. octohamatus was investigated by exposing a sympatric rhinobatid host species, the western shovelnose ray, Aptychotrema vincentiana, to B. octohamatus larvae newly emerged from eggs laid by adult parasites from gills of T. fasciata. Experimental exposure of A. vincentiana to freshly hatched B. octohamatus larvae resulted in a persistent infection, indicating that B. octohamatus may not be strictly host specific. Post-larval development charted on these experimentally infected A. vincentiana specimens was slow. Parasites appeared to be sexually mature at 91 days at 21–25°C. Branchotenthes octohamatus larvae bear only 4 pairs of hooklets on the haptor whereas all other hexabothriid larvae described so far have 5 hooklet pairs. Ontogenetic changes to the haptor revealed that it is probably hooklet pair III that is lost from B. octohamatus prior to larval development.
dc.description.statementofresponsibilityV. Glennon, L. A. Chisholm and I. D. Whittington
dc.identifier.citationParasitology (Cambridge), 2007; 134(9):1243-1252
dc.identifier.doi10.1017/S0031182007002545
dc.identifier.issn0031-1820
dc.identifier.issn1469-8161
dc.identifier.orcidChisholm, L. [0000-0002-4431-1455]
dc.identifier.urihttp://hdl.handle.net/2440/44414
dc.language.isoen
dc.provenancePublished online by Cambridge University Press 23 Mar 2007
dc.publisherCambridge Univ Press
dc.rightsCopyright © Cambridge University Press 2007
dc.source.urihttps://doi.org/10.1017/s0031182007002545
dc.subjectinfection
dc.subjectlarvae
dc.subjectdevelopment
dc.subjectCFSE
dc.subjectBranchotenthes octohamatus
dc.subjectHexabothriidae
dc.subjectMonogenea
dc.subjectRhinobatidae
dc.subjectspecificity
dc.titleExperimental infections, using a fluorescent marker, of two elasmobranch species by unciliated larvae of Branchotenthes octohamatus (Monogenea: Hexabothriidae): invasion route, host specificity and post-larval development
dc.typeJournal article
pubs.publication-statusPublished

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
hdl_44414.pdf
Size:
248.56 KB
Format:
Adobe Portable Document Format
Description:
Published version
Download

Collections

Earth and Environmental Sciences publications
Environment Institute publications