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Identification, functional characterization, and chromosomal localization of USP15, a novel human ubiquitin-specific protease related to the UNP oncoprotein, and a systematic nomenclature for human ubiquitin-specific proteases

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dc.contributor.authorBaker, R.
dc.contributor.authorWang, X.W.
dc.contributor.authorWoollatt, E.
dc.contributor.authorWhite, J.
dc.contributor.authorSutherland, G.
dc.date.issued1999
dc.description.abstractWe have identified a novel gene, USP15, encoding a human ubiquitin-specific protease (USP). The USP15 protein consists of 952 amino acids with a predicted molecular mass of 109.2 kDa and contains the highly conserved Cys and His boxes present in all members of the UBP family of deubiquitinating enzymes. USP15 shares 60.5% sequence identity and 76% sequence similarity with the human homolog (UNP/Unph/USP4) of the mouse Unp proto-oncogene. Recombinant USP15 demonstrated ubiquitin-specific protease activity against engineered linear fusions of ubiquitin to beta-galactosidase and glutathione S-transferase. USP15 can also cleave the ubiquitin-proline bond, a property previously unique to Unp/UNP. Chromosomal mapping by fluorescence in situ hybridization and radiation hybrid analyses localized the USP15 gene to chromosome band 12q14, a different location than that of UNP (3p21.3). Analysis of expressed sequence tag databases reveals evidence of alternate polyadenylation sites in the USP15 gene and also indicates that the gene may possess an exon/intron structure similar to that of the Unp gene, suggesting they have descended from a common ancestor. A systematic nomenclature for the human USPs is proposed.
dc.description.statementofresponsibilityRohan T. Baker, Xiao-Wen Wang, Erica Woollatt, Julia A. White, Grant R. Sutherland
dc.identifier.citationGenomics, 1999; 59(3):264-274
dc.identifier.doi10.1006/geno.1999.5879
dc.identifier.issn0888-7543
dc.identifier.issn1089-8646
dc.identifier.urihttp://hdl.handle.net/2440/7270
dc.language.isoen
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE
dc.source.urihttps://doi.org/10.1006/geno.1999.5879
dc.subjectChromosomes, Human, Pair 12
dc.subjectAnimals
dc.subjectHumans
dc.subjectMice
dc.subjectUbiquitin Thiolesterase
dc.subjectEndopeptidases
dc.subjectProline
dc.subjectAvian Proteins
dc.subjectDNA-Binding Proteins
dc.subjectTrans-Activators
dc.subjectOncogene Proteins
dc.subjectProto-Oncogene Proteins
dc.subjectTranscription Factors
dc.subjectUbiquitins
dc.subjectDNA, Complementary
dc.subjectChromosome Mapping
dc.subjectCloning, Molecular
dc.subjectAmino Acid Sequence
dc.subjectBase Sequence
dc.subjectSequence Homology, Amino Acid
dc.subjectMolecular Sequence Data
dc.subjectMafB Transcription Factor
dc.subjectTerminology as Topic
dc.subjectUbiquitin-Specific Proteases
dc.subjectProto-Oncogene Mas
dc.titleIdentification, functional characterization, and chromosomal localization of USP15, a novel human ubiquitin-specific protease related to the UNP oncoprotein, and a systematic nomenclature for human ubiquitin-specific proteases
dc.typeJournal article
pubs.publication-statusPublished

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