Molecular analysis of hybrids among the ornamental eucalypts Eucalyptus macrocarpa, E. pyriformis, and E. youngiana

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dc.contributor.authorNeaylon, K.
dc.contributor.authorDelaporte, K.
dc.contributor.authorSedgley, M.
dc.contributor.authorCollins, G.
dc.contributor.authorConran, J.
dc.date.issued2001
dc.descriptionCopyright © 2001 American Society for Horticultural Science
dc.description.abstractThe potential for hybridization among three species of Eucalyptus L'Hér in the Series Macrocarpae, E. macrocarpa Hook (Mottlecah), E. pyriformis Turcz. (pear-fruited mallee), and E. youngiana F. Muell. (large-fruited mallee), was investigated using molecular data generated by randomly amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR) analysis. Samples of DNA from seedlings derived from controlled pollinations, and from different individuals from each species, were amplified with six different 10-mer primers. The presence or absence of RAPD fragments was used to generate a dendrogram based on genetic similarity, an ordination derived by multidimensional scaling (MDS), and a minimum spanning tree (MST) to show the relative links and dissimilarities between the individuals tested. Two clusters were identified on the unweighted pair-group method arithmetric average dendrogram. The first included all of the E. macrocarpa genotypes and all but one of the E. macrocarpa hybrids. The second included all of the E. youngiana and E. pyriformis genotypes and their hybrids. The MDS ordinations placed the hybrid seedlings between the parent species. From the 30 progeny investigated, 28 were assessed from the molecular data to be hybrids from controlled pollinations. The remaining two seedlings appeared to be derived from self-pollination. The parentage of two mature trees, thought to be natural hybrids involving the three species, was also investigated. One was confirmed as a cross between E. youngiana and E. pyriformis, but the second was less certain because of its low genetic similarity to all other individuals, and may be a hybrid involving species not included in this study.
dc.description.statementofresponsibilityKirsty Neaylon, Kate L. Delaporte, Margaret Sedgley and Graham G. Collins, John G. Conran
dc.identifier.citationJournal of the American Society for Horticultural Science, 2001; 126(3):336-339
dc.identifier.doi10.21273/jashs.126.3.336
dc.identifier.issn0003-1062
dc.identifier.issn2327-9788
dc.identifier.orcidDelaporte, K. [0000-0002-0633-3493]
dc.identifier.orcidConran, J. [0000-0003-2268-2703] [0009-0002-8116-4527]
dc.identifier.urihttp://hdl.handle.net/2440/13069
dc.language.isoen
dc.publisherAmer Soc Horticultural Science
dc.source.urihttp://journal.ashspublications.org/cgi/content/abstract/126/3/336
dc.subjectEucalyptus
dc.subjecthybrid paternity
dc.subjectRAPD-PCR
dc.subjectUPGMA
dc.subjectmultivariate analysis
dc.subjectDNA fingerprinting
dc.subjectgenetic similarity
dc.subjectmorph molecular RAPD seedling
dc.titleMolecular analysis of hybrids among the ornamental eucalypts Eucalyptus macrocarpa, E. pyriformis, and E. youngiana
dc.typeJournal article
pubs.publication-statusPublished

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