High-density lipoproteins neutralize C-reactive protein proinflammatory activity

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dc.contributor.authorWadham, C.
dc.contributor.authorAlbanese, N.
dc.contributor.authorRoberts, J.
dc.contributor.authorWang, L.
dc.contributor.authorBagley, C.
dc.contributor.authorGamble, J.
dc.contributor.authorRye, K.
dc.contributor.authorBarter, P.
dc.contributor.authorVadas, M.
dc.contributor.authorXia, P.
dc.date.issued2004
dc.description.abstractBackground— C-reactive protein (CRP), a well-recognized marker of atherosclerosis, has recently been suggested to have a direct proinflammatory effect. The constitutive expression of low levels of CRP in normal plasma suggests the likelihood that a natural factor exists to neutralize the effect of CRP. This factor(s) has not yet been identified. Method and Results— The proinflammatory effect of CRP was measured by the induction of inflammatory adhesion molecules in human umbilical vein endothelial cells (HUVECs). We show that CRP significantly induced upregulation of adhesion molecules in both protein and mRNA levels. The CRP-induced expression of these inflammatory adhesion molecules was completely suppressed when the cells were preincubated with a physiological concentration (1 mg/mL apolipoprotein A-I) of HDLs derived from human plasma (native HDL) or reconstituted HDL (rHDL) at a very low concentration (0.01 mg/mL apolipoprotein A-I). A novel mechanism of HDL inhibition is likely to operate, because (1) rHDL was 100 times more potent than native HDL, (2) preincubation with HDL and its sustained presence were obligatory, and (3) oxidized 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine was the fundamental active component. Conclusions— The CRP-induced upregulation of inflammatory adhesion molecules in HUVECs was completely prevented by HDL via their oxidized phospholipid components.
dc.identifier.citationCirculation, 2004; 109(17):2116-2122
dc.identifier.doi10.1161/01.CIR.0000127419.45975.26
dc.identifier.issn0009-7322
dc.identifier.issn1524-4539
dc.identifier.urihttp://hdl.handle.net/2440/9364
dc.language.isoen
dc.publisherLippincott Williams & Wilkins
dc.source.urihttps://doi.org/10.1161/01.cir.0000127419.45975.26
dc.subjectLipoproteins
dc.subjectprotein
dc.subjectinflammation
dc.subjectatherosclerosis
dc.titleHigh-density lipoproteins neutralize C-reactive protein proinflammatory activity
dc.typeJournal article
pubs.publication-statusPublished

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