Comparison of culture, histopathology and urease testing for the diagnosis of helicobacter pylori gastritis and susceptibility to amoxycillin, clarithromycin, metronidazole and tetracycline

Simple item page
dc.contributor.authorGrove, D.
dc.contributor.authorKoutsouridis, G.
dc.contributor.authorCummins, A.
dc.date.issued1998
dc.description.abstractGastric biopsy specimens were taken from 737 patients undergoing upper gastrointestinal endoscopy and assessed for Helicobacter pylori infection. The diagnostic utilities of H. pylori culture (733 patients), detection of urease production (724 patients) and histopathological examination (469 patients) were compared. Since each of these techniques may fail to diagnose patients infected with H. pylori, an attempt was made to estimate the true rate of infection using a mathematical approach that combined the results of culture, histopathology and urease testing; 34% of the 733 patients were thought to be infected. Using this figure as a benchmark, the sensitivity, specificity, positive predictive value and negative predictive value of H. pylori culture were 73.2%, 100%, 100% and 86.3%, respectively, compared with 58.7%, 100%, 100% and 89.6%, respectively for urease production and 77.0%, 100%, 100% and 82.4%, respectively for histopathology. Thus, histopathological examination was the single most reliable test. A combination of histopathological examination and H. pylori culture diagnosed 99.5% of patients that were estimated to be truly infected. The minimum inhibitory concentrations of a number of antibiotics were measured for 135 isolates of H. pylori. All isolates were susceptible to amoxycillin and tetracycline whereas 5.2% were resistant to clarithromycin and 60% were resistant to metronidazole.
dc.identifier.citationPathology, 1998; 30(2):183-187
dc.identifier.doi10.1080/00313029800169206
dc.identifier.issn0031-3025
dc.identifier.issn1465-3931
dc.identifier.orcidCummins, A. [0000-0003-3115-9498]
dc.identifier.urihttp://hdl.handle.net/2440/9117
dc.language.isoen
dc.publisherElsevier BV
dc.source.urihttps://doi.org/10.1080/00313029800169206
dc.subjectStomach
dc.subjectHumans
dc.subjectHelicobacter pylori
dc.subjectGastritis
dc.subjectAmoxicillin
dc.subjectClarithromycin
dc.subjectMetronidazole
dc.subjectTetracycline
dc.subjectUrease
dc.subjectAnti-Bacterial Agents
dc.subjectAntitrichomonal Agents
dc.subjectBiopsy
dc.subjectMicrobial Sensitivity Tests
dc.subjectSensitivity and Specificity
dc.subjectPredictive Value of Tests
dc.titleComparison of culture, histopathology and urease testing for the diagnosis of helicobacter pylori gastritis and susceptibility to amoxycillin, clarithromycin, metronidazole and tetracycline
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Medicine publications